HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Román-Avilés, B. Articles by Kelly, J. D. Search for Related Content PubMed Articles by Román-Avilés, B. Articles by Kelly, J. D. Agricola Articles by Román-Avilés, B. Articles by Kelly, J. D. Related Collections Other Legumes Plant Disease Crop Genetics

GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY

Identification of Quantitative Trait Loci Conditioning Resistance to Fusarium Root Rot in Common Bean

Dep. of Crop and Soil Sciences, Michigan State Univ., East Lansing, MI 48824

^* Corresponding author (kellyj{at}msu.edu)

Fusarium root rot [caused by the soil borne pathogen Fusarium solani (Mart.) Appel & Wr. f. sp. phaseoli (Burk.) Snyd. & Hans.] is a major constraint to common bean (Phaseolus vulgaris L.) production worldwide. The objectives of this study were to transfer Fusarium root rot resistance from small-seeded Middle American black bean into highly susceptible large-seeded Andean kidney and cranberry bean genotypes and identify quantitative trait loci (QTL) associated with Fusarium root rot resistance in common bean. Two inbred backcross line (IBL) populations, developed from crosses between small-seeded resistant and large-seeded genotypes, were evaluated for reaction to root rot during 2 yr in field and greenhouse experiments. Significant genetic variation that displayed continuous transgressive segregation toward root rot susceptibility confirmed the quantitative nature of resistance. Highly significant treatment-by-environmental effects were observed. Narrow-sense heritability estimates for resistance ranged from 0.10 to 0.51 for the kidney and from 0.20 to 0.82 for the cranberry IBL populations. Nine QTL significantly associated with Fusarium root rot resistance in the field and greenhouse, explained from 5 to 53% of the total phenotypic variability. The QTL associated with root rot resistance were located on linkage groups (LGs) B2 and B5 of the integrated bean map close to previously identified QTL for resistance on B2. On the basis of a linear regression model, a combination of five markers associated with QTL on two different LGs accounted for 73% of the phenotypic variation for root rot resistance. Data from the current study provides breeders the opportunity to combine, through marker-assisted backcrossing, large-effect QTL identified on different LGs to enhance root rot resistance in Andean germplasm of common bean.

Abbreviations: BJ, BAT93 x Jalo EEP558 recombinant inbred line population • CIM, composite interval mapping • cM, centimorgan • DTF, days to flower • h²_N, narrow-sense heritability • IBL, inbred backcross line • LG, linkage group • LOD, log of odds • MRF, Montcalm Research Farm • NSL, Negro San Luís • NYSAES, New York State Agriculture Experimental Station • QTL, quantitative trait loci • RAPD, random amplified polymorphic DNA • RIL, recombinant inbred line • SMA, single marker analysis

This article has been cited by other articles:

				F. Navarro, M. E. Sass, and J. Nienhuis Identification and Confirmation of Quantitative Trait Loci for Root Rot Resistance in Snap Bean Crop Sci., May 1, 2008; 48(3): 962 - 972. [Abstract] [Full Text] [PDF]

				J. A. Acosta-Gallegos, J. D. Kelly, and P. Gepts Prebreeding in Common Bean and Use of Genetic Diversity from Wild Germplasm Crop Sci., December 18, 2007; 47(Supplement_3): S-44 - S-59. [Abstract] [Full Text] [PDF]

				M. Ender and J. D. Kelly Identification of QTL Associated with White Mold Resistance in Common Bean Crop Sci., October 27, 2005; 45(6): 2482 - 2490. [Abstract] [Full Text] [PDF]