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Published online 23 February 2005
Published in Crop Sci 45:735-739 (2005)
© 2005 Crop Science Society of America
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GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY

Screening Sunflower for Resistance to Sunflower chlorotic mottle virus and Mapping the Rcmo-1 Resistance Gene

S. L. Lenardona,*, M. E. Bazzalob,1, G. Abrattib, C. J. Cimminob, M. T. Galellab, M. Grondonab, F. Giolittia and A. J. Leónb

a IFFIVE-CICVA, INTA. Camino 60 Cuadras Km 5 1/2 X5020ICA, Córdoba, Argentina
b ADVANTA SEMILLAS SAIC Ruta 226 Km 60,5 (7620) Balcarce, Buenos Aires, Argentina

* Corresponding author (slenard{at}infovia.com.ar)

Sunflower chlorotic mottling is an emerging virus disease which has been detected in commercial hybrids and wild sunflowers in Argentina. Two hundred thirty-two sunflower (Helianthus annuus L.) lines were screened for resistance to Sunflower chlorotic mottle virus (SuCMoV) using artificial inoculations under greenhouse conditions. Only three lines (L33, L74, and L52) showed a partial resistance, L33 being the most resistant. Virus replication was delayed (A405 in DAS-ELISA) and morphological traits (plant height and leaf width) were less affected in this line and its various hybrid combinations than in susceptible controls. Segregation data from an artificially inoculated F2 population, under greenhouse and field conditions, indicated the presence of a single dominant resistance gene which was designated Rcmo-1. This gene was mapped on Linkage Group 14 between markers MS0022 (5 cM) and ORS-307 (4 cM). Linkage between the two markers and the Rcmo-1 locus was confirmed in a field evaluation.

Abbreviations: CM, chlorotic mottling • DAS-ELISA, double antibody sandwich-enzyme linked inmunosorbent assay • ICP, isolated chlorotic pinpoint • MM, mild mosaic • SCM, severe chlorotic mottling • SCP, scarce chlorotic pinpoint • SuCMoV, Sunflower chlorotic mottle virus


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Crop Science 2005 45: vi. [Full Text]  






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