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Published online 23 February 2005
Published in Crop Sci 45:717-721 (2005)
© 2005 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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CROP BREEDING, GENETICS & CYTOLOGY

Introgression of Perennial Teosinte Genome into Maize and Identification of Genomic In Situ Hybridization and Microsatellite Markers

Qilin Tanga, Tingzhao Ronga,*, Yunchun Songb, Junpin Yangc, Guangtang Pana, Wanchen Lia, Yubi Huanga and Moju Caoa

a Maize Research Inst., Sichuan Agriculture Univ., Ya'an, Sichuan 625014, P.R. China
b The Key Lab. of the Ministry of Education of Plant Developmental Biology, Wuhan Univ., Wuhan, 430072, P.R. China
c Sichuan Crop Research Inst., Chengdu, 625000, P.R. China

* Corresponding author (rongtz{at}sicau.edu.cn)

To transfer desirable characters from perennial teosinte [Zea perennis (Hitchc.) Reeves & Mangelsd.] into maize (Z. mays L.), we have generated an interspecific hybrid and its backcross generations (BC1F3). The maize x perennial teosinte BC1F3 (MZI202) resembled maize, with chromosome number 2n = 20. MZI202 was studied by multicolor genomic in situ hybridization (McGISH) and simple-sequence repeat (SSR) microsatellites markers. The McGISH experiments provided direct evidence that MZI202 was a maize–perennial teosinte substitution line with introgression of three alien chromosomes from perennial teosinte. The SSR assay further confirmed that a single chromosome 6 and the pair of chromosome 10 of maize were replaced by perennial teosinte chromosomes in MZI202.

Abbreviations: DAPI, 4',6-diamidino-2-phenylindole • GISH, genomic in situ hybridization • McGISH, multicolor genomic in situ hybridization • PBS, phenobarbital sodium • PCR, polymerase chain reaction • SSC, standard saline citrate • SSR, simple-sequence repeat






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