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SYMPOSIUM—GENETIC AND METABOLIC ENGINEERING FOR VALUE-ADDED TRAITS

Engineering Soybean for Enhanced Sulfur Amino Acid Content

Plant Genetics Research Unit, Agricultural Research Service-USDA and Department of Agronomy, University of Missouri, Columbia, MO 65211

^* Corresponding author (KrishnanH{at}missouri.edu)

Soybean [Glycine max (L.) Merr.] is an important protein source in human diets and animal rations. Additionally, human consumption of soybean protein is reported to provide specific health benefits. Since methionine and cysteine contents limit the nutritional value of soybean, efforts involving both traditional breeding and genetic engineering have been employed in attempts to increase the presence of these essential amino acids. Traditional breeding has been primarily utilized to increase the total protein content but not to enhance the sulfur amino acid content of soybean. Although mutagenesis in conjunction with traditional breeding is a viable approach for enhancing the sulfur amino acid content of soybean, genetic engineering appears to be a more realistic approach. Introduction of methionine-rich heterologous proteins has resulted in a modest increase of this amino acid in soybean. Either elevating the expression of endogenous methionine-rich proteins or introducing synthetic proteins containing a high percentage of essential amino acids are other possible approaches that may increase the nutritional quality of the seed. Even though considerable progress has been made in enhancing the methionine content of soybean, several obstacles remain. A thorough understanding the sulfur assimilatory pathway in soybean is a prerequisite for improving the sulfur amino acid content. Expression of feedback-insensitive forms of serine acetyl transferase and O-acetylserine (thiol) lyase, two key enzymes in sulfur assimilatory pathway, could lead to an increase in the availability of sulfur amino acids. An adequate supply of sulfur amino acids in developing seeds may facilitate accumulation of sulfur-rich proteins to a level sufficient to meet the nutritional requirement of livestock and poultry.

Abbreviations: BNA, Brazil nut albumin • HVR, hypervariable region • MRP, methionine-rich protein • PDCAAS, protein digestibility corrected amino acid score • SAT, serine acetyltransferase

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