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Published in Crop Sci. 44:2063-2067 (2004).
© 2004 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA

CROP BREEDING, GENETICS & CYTOLOGY

Origin of the Blue-Aleurone Gene in Sebesta Blue Wheat Genetic Stocks and a Protocol for Its Use in Apomixis Screening

L. A. Morrisona, R. J. Metzgerb,* and A. J. Lukaszewskic

a Herbarium, Dep. of Botany and Plant Pathology, Oregon State Univ., Corvallis, OR 97331-2910
b Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR 97331-3002
c Dep. of Botany and Plant Sciences, University of California, Riverside, CA 92521

* Corresponding author (bob.metzger{at}oregonstate.edu)

Tracing the pedigree of the blue aleurone locus in the Sebesta Blue wheat (Triticum aestivum L.) genetic stocks has proven difficult. There is limited information on the original intergeneric crosses that moved the blue-aleurone trait from Thinopyrum ponticum (Podp.) Z.W. Liu & R.-C. Wang into wheat. Blue Baart, an unregistered genetic stock developed at the University of California-Davis, was the direct parental source for the blue-aleurone trait in Sebesta Blue. Its wheat pedigree is unclear because of the introgressing, bulk population technique by which it was developed from the original intergeneric wheat x Thinopyrum cross. In situ probing with total genomic DNA in the spring and winter Sebesta Blue lines showed a 4EL Thinopyrum translocation and numerous unidentified chromosomal rearrangements in the two winter lines. A protocol is presented for use of the Sebesta Blue genetic stocks for apomixis studies.

Abbreviations: PBB, Pugsley's Male Sterile/Blue Baart • SB, Sebesta Blue




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R.J. Metzger and E. Sebesta
Registration of Three Blue-Seeded Wheat Genetic Stocks Exhibiting Xenia
Crop Sci., November 1, 2004; 44(6): 2281 - 2282.
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