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GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY

Screening Multiple Soybean Cultivars (MG 00 to MG VIII) for Somatic Embryogenesis Following Agrobacterium-Mediated Transformation of Immature Cotyledons

^a Dep. of Natural Resources and Environmental Sciences, Univ. of Illinois, Urbana, IL 61801
^b USDA-ARS, Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, Dep. of Crop Sciences, Univ. of Illinois, Urbana, IL 61801

^* Corresponding author (korban{at}uiuc.edu).

A total of 15 soybean [Glycine max (L.) Merr.] cultivars representing maturity groups 00 to VIII were assessed for their embryogenic and transformation potentials. All cultivars were identified as embryogenic under hygromycin selection following Agrobacterium-mediated transformation of immature cotyledons. Histochemical ß-glucuronidase (GUS) assays of induced hygromycin-resistant somatic embryos (SEs) showed that 13 out of 15 cultivars were amenable for transformation. Wide variations among different genotypes were observed for their embryogenic capacity under hygromycin selection. Three cultivars, Cisne, Council, and Kunitz, were highly embryogenic yielding more than 50% responding explants and 1.5 to 2.4 hygromycin-resistant SEs per responding explant. The transformation potential of multiple soybean cultivars was highly correlated with the embryogenic potential of immature cotyledons under hygromycin selection. It was possible to distinguish between highly and poorly embryogenic genotypes by visually observing the phenotype of cultured immature cotyledons. For highly embryogenic cultivars, the induction of somatic embryos mainly originated from actively dedifferentiating and browning/necrotic tissues along the margins of the abaxial side of cultured cotyledons.

Abbreviations: 2,4-D, 2,4-dichlorophenoxyacetic acid • GUS, ß-Glucuronidase • HPT, Hygromycin phosphotransferase • SEs, somatic embryos

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Crop Science 2004 44: 1507-1510. [Full Text]  

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