HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Related articles in Crop Science Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Tuna, M. Articles by Arumuganathan, K. Search for Related Content PubMed Articles by Tuna, M. Articles by Arumuganathan, K. Agricola Articles by Tuna, M. Articles by Arumuganathan, K. Related Collections Crop Cytology Crop Genetics Other Forage Crops

CROP BREEDING, GENETICS & CYTOLOGY

C-Banding Analyses of Bromus inermis Genomes

^a Dep. of Field Crops, Tekirdag Agric. Faculty, Univ. of Trakya, Tekirdag, Turkey
^b USDA-ARS, Wheat, Sorghum, and Forages Res. Unit, 344 Keim Hall, Univ. of Nebraska, P.O. Box 830937, Lincoln, NE 68507-0937
^c Dep. of Crop and Soil Sci., Washington State Univ., Pullman, WA 99164-6420
^d Flow and Image Cytometry Core Laboratories, Benaroya Research Inst. at Virginia Mason, 1201 Ninth Ave., Seattle, WA 98101

^* Corresponding author (kpv{at}unlserve.unl.edu).

Smooth bromegrass (Bromus inermis Leyss.) has both tetraploid (2n = 28) and octaploid (2n = 56) ploidy levels that have been difficult to characterize cytogenetically because of similar chromosome morphology. Objectives of this study were to identify individual chromosomes of tetraploid and octaploid B. inermis with C-banding procedures along with chromosome length and arm length ratios, develop more detailed karyotypes than those previously available, and use the karyotypes to examine the genomic relationship of tetraploid and octaploid B. inermis. Root tips of the plants from four tetraploid and three octaploid accessions were used to produce chromosome squash preparations for cytogenetic analysis. The tetraploid B. inermis genome consisted of 12 chromosomes with a telomeric band on each arm and sixteen chromosomes with only one telomeric band on one arm. All of the chromosomes of the tetraploid form, except for four chromosomes, were identified by C-banding patterns, chromosome length, and arm length ratio. The octaploid B. inermis genome consisted of four chromosomes with no C-bands, 14 chromosomes with two telomeric bands, and 38 chromosomes with only one telomeric band on either the short or long arm. The combined use of C-banding, chromosome size, and arm length ratio only enabled groups of 2, 4, 6, or 8 similar chromosomes to be identified because of similarities in chromosome morphology of the octaploids. Results indicate that tetraploid B. inermis is an allotetraploid since all chromosomes except four could be separated into identifiable pairs. Because of differences between expected and actual numbers of satellite chromosomes and chromosomes with specific C-banding patterns, octaploid B. inermis is probably not a doubled form of the tetraploid B. inermis.

Abbreviations: GISH, genomic in situ hybridization • NOR, nucleolus organizer region

Related articles in Crop Science:

THIS ISSUE IN CROP SCIENCE

Crop Science 2004 44: 1-4. [Full Text]  

This article has been cited by other articles:

				M. Tuna, K. P. Vogel, and K. Arumuganathan Cytogenetic and Nuclear DNA Content Characterization of Diploid Bromus erectus and Bromus variegatus Crop Sci., February 1, 2006; 46(2): 637 - 641. [Abstract] [Full Text] [PDF]