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PLANT GENETIC RESOURCES

AFLP Variation in Four Blue Grama Seed Sources

^a Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, S7N 0X2, Canada
^b Native Plant Solutions, Ducks Unlimited Canada, 1255 Clarence Avenue, Winnipeg, MB, R3T 1T4, Canada

^* Corresponding author (fuy{at}agr.gc.ca).

Blue grama [Bouteloua gracilis (Willd. ex Kunth) Lag. ex Griffiths] is one of the most widespread native grasses in western North America. Several blue grama seed sources are currently used for rangeland seeding, but little is known about the genetic diversity of these seed sources. Amplified fragment length polymorphism (AFLP) technique was applied to compare the genetic diversity among four blue grama seed sources (a precultivar germplasm [balanced multisite composite, BMSC], the ecotype Bad River, a Minnesota ecotype, and a native Manitoba seed collection) and to assess the genetic shift during two generations of BMSC seed multiplication. Germplasm BMSC was a balanced multisite composite of 99 clones selected from 495 live plants collected from 11 sites across Manitoba. Six AFLP primer pairs were employed to screen a total of 176 individual plants sampled from both the first three generations of BMSC and the other three seed sources and 167 polymorphic AFLP bands were scored for each plant. Large AFLP variation was observed within the four seed sources. Greater AFLP variation was detected in the BMSC than Bad River, Minnesota ecotype, and the Manitoba native harvest. No genetic shift in the BMSC was found across the two seed multiplications. These results indicate a balanced composite of multisite blue grama germplasm can maintain high genetic diversity with little genetic shift in a few generations of seed multiplication.

Abbreviations: AFLP, amplified fragment length polymorphism • AMOVA, analysis of molecular variance • BMSC, balanced multisite composite

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Crop Science 2004 44: 1-4. [Full Text]  

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