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TURFGRASS SCIENCE

Estimation of Pollen Viability, Shedding Pattern, and Longevity of Creeping Bentgrass on Artificial Media

^a Dep. of Horticulture, 257 Horticulture Hall, Iowa State Univ., Ames, IA 50011
^b The Scotts Co., 14111 Scottslawn Rd., Marysville, OH 43041

^* Corresponding author (sfei{at}iastate.edu).

An estimation of pollen viability is needed to determine pollen daily shedding pattern and longevity. Both parameters provide valuable information for plant breeding and contribute to the risk assessment of pollen-producing transgenic crops. Pollen viability of ‘Crenshaw’ creeping bentgrass (Agrostis stolonifera L.) was estimated through pollen germination on media containing sucrose (1-alpha-D-glucopyranosyl-2-beta-D-fructofranoside, at 0.25, 0.5, 1.0 M), H₃BO₃ (1.0, 2.0, or 4.0 mM), and CaCl₂ (1.0, 2.0, or 4.0 mM). The highest pollen germination percentage was obtained on a medium containing 1.0 M sucrose, 1.0 mM H₃BO₃, and 2.0 mM CaCl₂. Concentrations of all three medium components had significant effects on pollen germination. No two-way or three-way interactions among sucrose, H₃BO₃, and CaCl₂ were observed. A high sucrose concentration of 1.0 M severely inhibited pollen tube growth, causing shorter and thicker pollen tubes. Pollen-tube length of pollen germinated on medium containing 1.0 M sucrose averaged 137 µm, while those germinated on medium containing 0.5 M sucrose averaged 248 µm. The daily shedding pattern of pollen of Crenshaw creeping bentgrass was determined by collecting and germinating pollen from 0800 through 1700 h at 1-h intervals. The results indicated there were two peaks of pollen viability, with the first one occurring at 0900 h and the second peak at 1400 h. The longevity of Crenshaw and ‘Penncross’ pollen was assessed by storing the pollen in a desiccator at 21°C and a relative humidity of 64 to 66%. Our results showed that creeping bentgrass pollen lost viability dramatically within the first 1.5 h of storage and lost viability completely after 3 h of storage.

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