HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Tang, H. V. Articles by Pring, D. R. Search for Related Content PubMed Articles by Tang, H. V. Articles by Pring, D. R. Agricola Articles by Tang, H. V. Articles by Pring, D. R. Related Collections Sorghum Plant Genetic Resources Crop Genetics

CROP BREEDING, GENETICS & CYTOLOGY

Conversion of Fertility Restoration of the Sorghum IS1112C (A3) Male-Sterile Cytoplasm from Two Genes to One Gene

USDA-ARS, Crop Genetics and Environment Unit, Department of Plant Pathology, 1453 Fifield Hall, University of Florida, Gainesville, FL 32611 USA

^* Corresponding author (drpg{at}mail.ifas.ufl.edu).

The restoration of male fertility in sorghum [Sorghum bicolor (L.) Moench] lines carrying the IS1112C male-sterile cytoplasm is characterized by limited seed set. Restoration requires complementary gametophytic action of two restoring alleles, Rf3 and Rf4, for individual gamete viability, and an F₁ heterozygous for the two restoring loci is expected to exhibit 25% viable pollen. The objective of this study was to demonstrate the feasibility of converting restoration to single gene behavior. Single-seed descent B3Tx398/IS1112C F_5:6 lines were used to generate homozygosity at the rf3 and rf4 loci, and critical segregants were identified by progeny tests, assays for action of the Rf3 allele, and genomic markers for the rf4 locus. Using these criteria, we constructed the genotypes Rf3Rf3rf4rf4 and rf3rf3Rf4Rf4 in normal, male-fertile cytoplasm lines, and in IS1112C male-sterile cytoplasm lines. Pollination of either male-sterile line with a matching male-fertile line resulted in about 25% pollen staining in the F₁, demonstrating complementation of the two restoring loci. Pollination of either male-sterile line with IS1112C, Rf3Rf3Rf4Rf4, resulted in about 50% pollen staining. These characteristics substantiate complementary action of the restoring alleles, and are consistent with successful conversion to a single gene fertility restoration system.

This article has been cited by other articles:

				H. V. Tang, J. F. Pedersen, C. D. Chase, and D. R. Pring Fertility Restoration of the Sorghum A3 Male-Sterile Cytoplasm through a Sporophytic Mechanism Derived from Sudangrass Crop Sci., May 31, 2007; 47(3): 943 - 950. [Abstract] [Full Text] [PDF]

				L. C. Kuhlman, D. R. Pring, W. L. Rooney, and H. V. Tang Allelic Frequency at the Rf3 and Rf4 Loci and the Genetics of A3 Cytoplasmic Fertility Restoration in Converted Sorghum Lines Crop Sci., May 18, 2006; 46(4): 1576 - 1580. [Abstract] [Full Text] [PDF]