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CROP BREEDING, GENETICS & CYTOLOGY

Translocation Breakpoints in Soybean Classical Genetic Linkage Groups 6 and 8

USDA-ARS-CICGR and Dep. of Agronomy and Zoology/Genetics, Iowa State Univ., Ames, IA 50011

^* Corresponding author (rpalmer{at}iastate.edu).

Reciprocal chromosome translocations are important for locating genes to linkage groups (LGs). Identifying the chromosomes involved in translocations is necessary for the isolation of tester sets. Our objectives were (i) to determine the location of translocation breakpoints by testing linkage with loci of Classical Linkage Group (CLG) 6 (Df₂ and Y₁₁), CLG 8 (Adh₁, Ms₁, Wm, Ms₆, St₅, W₁, and Y₂₃), and other CLGs, and (ii) to confirm the orientation of these nine marker loci. The ‘KS172-11-3’, ‘KS175-7-3’, ‘Clark T/T’, ‘KS171-31-2’, PI 189866, and ‘L75-0283-4’ soybean lines with homozygous chromosome translocations were crossed to the same genetic marker types. F₂ seed was increased at the University of Puerto Rico/Iowa State University soybean nursery near Isabela, PR. Data for the different characters used as marker traits were collected from F₂ populations and F_2:3 families. Recombination values revealed linkage between the breakpoints in KS172-11-3, KS175-7-3, and Clark T/T, with Df₂, Y₁₁, and several loci of CLG 8. Interestingly, these three translocations had a common breakpoint between Y₁₁ and Ms₁, but no linkage was identified between these loci and the breakpoints in KS171-31-2, PI 189866, and L75-0283-4. Our data further showed that CLGs 6 and 8 are the same LG, with Df₂ and Adh₁ at the ends of the chromosome segment. KS172-11-3, KS175-7-3, and Clark T/T share a common translocated chromosome which is different from that in KS171-31-2, PI 189866, and L75-0283-4. This information will facilitate the assignment of CLGs and the isolation of a tester set of translocations, and enhance genetic linkage mapping.

Abbreviations: LG, Linkage Group • CLG, Classical Linkage Group • MLG, Molecular Linkage Group