HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Diaby, M. Articles by Casler, M. D. Search for Related Content PubMed Articles by Diaby, M. Articles by Casler, M. D. Agricola Articles by Diaby, M. Articles by Casler, M. D. Related Collections Other Forage Crops Crop Genetics

PLANT GENETIC RESOURCES

RAPD Marker Variation among Smooth Bromegrass Cultivars

^a Department of Agronomy, University of Wisconsin-Madison, WI 53706-1597
^b USDA-ARS, U.S. Dairy Forage Research Center, 1925 Linden Dr. West, Madison, WI 53706-1108

^* Corresponding author (mdcasler{at}facstaff.wisc.edu)

The level of genetic diversity within and among smooth bromegrass (Bromus inermis Leyss.) cultivars and land races is unknown. The objective of this study was to investigate and characterize genetic diversity of smooth bromegrass cultivars and selected populations on the basis of random amplified polymorphic DNA (RAPD) markers. Variation among 277 individual plants from 40 smooth bromegrass cultivars was evaluated by means of RAPD markers. Nineteen primers evaluated amplified 97 informative amplicons. Several RAPD marker bands showed unique patterns of mean frequency differences among germplasm groups. A dendrogram constructed from average linkage cluster analysis did not indicate any clear pattern of division on the basis of discrete or putative climatype or adaptation zones. There was considerable correspondence to known pedigree relationships revealed from a previous smooth bromegrass morphological clustering analysis, particularly for lines that are closely related to each other. The two interspecific hybrids between B. inermis and B. pumpellianus Schribner (Polar) and B. riparius Rehm. (S-9183-H) did not exhibit any species-specific markers. All groups of smooth bromegrass germplasm were found to have high within-population genetic variation that ranged from 84 to 96% of the total, reflecting the outcrossing reproduction and probably the complex inheritance of smooth bromegrass. Analysis of molecular variance showed the largest interpopulation genetic variation for contemporary germplasm sources, supporting morphological studies for the existence of genetic variability among contemporary smooth bromegrass germplasms. These results suggest that landrace cultivars likely remain useful for germplasm improvement and cultivar development.

This article has been cited by other articles:

				M. A. R. Mian, J. C. Zwonitzer, Y. Chen, M. C. Saha, and A. A. Hopkins AFLP Diversity within and among Hardinggrass Populations Crop Sci., October 27, 2005; 45(6): 2591 - 2597. [Abstract] [Full Text] [PDF]

				A. B. Hollman, J. C. Stier, M. D. Casler, G. Jung, and L. A. Brilman Identification of Putative Velvet Bentgrass Clones Using RAPD Markers Crop Sci., March 28, 2005; 45(3): 923 - 930. [Abstract] [Full Text] [PDF]

				M. Diaby and M. D. Casler RAPD Marker Variation among Divergent Selections for Fiber Concentration in Smooth Bromegrass Crop Sci., January 1, 2005; 45(1): 27 - 35. [Abstract] [Full Text] [PDF]