HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Chung, J. Articles by Specht, J. E. Search for Related Content PubMed Articles by Chung, J. Articles by Specht, J. E. Agricola Articles by Chung, J. Articles by Specht, J. E. Related Collections Soybean Cell Biology & Molecular Genetics Crop Genetics

CELL BIOLOGY & MOLECULAR GENETICS

The Seed Protein, Oil, and Yield QTL on Soybean Linkage Group I

^a Dep. of Agronomy, Agric. College, GyeongSang National Univ., 900 Gaza-Dong, Chinju City, Geyong-Nam, South Korea, 660-701
^b Botany Dep., Iowa State Univ., Ames, IA 50011
^c Dep. of Agronomy & Horticulture, Univ. of Nebraska, Lincoln, NE 68583-0915
^d USDA, ARS, Bldg. 006, Room 100, BARC-West, 10300 Baltimore Ave., Beltsville, MD
^e USDA, ARS, Corn Insect and Crop Genetics Research Unit, Dep. of Agronomy, Iowa State Univ., Ames, IA 50011

^* Corresponding author (jspecht1{at}unl.edu)

Soybean [Glycine max (L.) Merr.] seed protein is negatively correlated with seed oil and often with yield. Our goal was to examine the basis for these correlations at a quantitative genetic locus (QTL) level. Seventy-six F₅–derived recombinant inbred lines (RILs) from the mating of the high-protein (480 g protein per kg seed) G. max accession PI 437088A with the high-yield cultivar Asgrow A3733 (420 g kg^-1 seed protein content) were evaluated in six irrigation treatments (i.e., 100, 80, 60, 40, 20, and 0% replacement of weekly evapotranspiration loss) of a two-replicate experiment conducted for 2 yr. The RILs were genotyped with 329 random amplified polymorphic DNAs (RAPDs), 103 simple sequence repeats (SSRs), and four other markers, creating a 2943-centimorgan (cM) genetic map of 35 linkage group (LGs) that, on the basis of SSR homology, aligned with the 20 known soybean LGs. The phenotypic regression of RIL protein and oil on yield revealed respective linear coefficients of -2.6 and +1.6 percentage points per kg ha^-1 (a protein/oil exchange ratio of -1.6). A seed protein, oil, and yield QTL mapped close to RAPD marker OPAW13a in a small LG-I interval that was flanked by the SSR markers Satt496 and Satt239. The additive effects of the PI 437088A allele on seed protein, oil, and yield were a respective +1.0 and -0.6 percentage points (a protein/oil ratio of -1.6) and -154 kg ha^-1. Given that the genetic-based protein/oil exchange ratio of 1.6 is smaller than the 2.0 calorific-based oil/protein ratio, one might expect the remaining 0.4 units of carbon and/or energy to be made available for other seed dry matter. However, yield almost invariably falls when seed protein is genetically enhanced at the expense of seed oil, suggesting that protein synthesis and its deposition in the seed is energetically more costly to the plant than is commonly assumed.

Abbreviations: AFLP(s), amplified fragment length polymorphism(s) • BC, backcross • BG, background • bp, base pair • CAPS, cleaved amplified polymorphic sequence • CIM, composite interval mapping • cM, centimorgan • DP, donor parent • LG, linkage group • LOD, base-10 logarithm of the odds ratio (i.e., likelihood of proposed model versus null model) • LR, linear regression • MG, maturity group • PI, plant introduction • QTL(s), quantitative trait locus (loci) • RAPD(s), random amplified polymorphic DNA(s) • RFLP(s), restriction fragment length polymorphism(s) • RIL(s), recombinant inbred line(s) • RP, recurrent parent • SIM, simple interval mapping • SSR(s), simple sequence repeat(s)

This article has been cited by other articles:

				M. A. Schmidt and E. M. Herman Suppression of Soybean Oleosin Produces Micro-Oil Bodies that Aggregate into Oil Body/ER Complexes Mol Plant, September 15, 2008; (2008) ssn049v1. [Abstract] [Full Text] [PDF]

				J. Burstin, P. Marget, M. Huart, A. Moessner, B. Mangin, C. Duchene, B. Desprez, N. Munier-Jolain, and G. Duc Developmental Genes Have Pleiotropic Effects on Plant Morphology and Source Capacity, Eventually Impacting on Seed Protein Content and Productivity in Pea Plant Physiology, June 1, 2007; 144(2): 768 - 781. [Abstract] [Full Text] [PDF]

				R. M.P. Siloto, K. Findlay, A. Lopez-Villalobos, E. C. Yeung, C. L. Nykiforuk, and M. M. Moloney The Accumulation of Oleosins Determines the Size of Seed Oilbodies in Arabidopsis PLANT CELL, August 1, 2006; 18(8): 1961 - 1974. [Abstract] [Full Text] [PDF]

				D. M. Nichols, K. D. Glover, S. R. Carlson, J. E. Specht, and B. W. Diers Fine Mapping of a Seed Protein QTL on Soybean Linkage Group I and Its Correlated Effects on Agronomic Traits Crop Sci., February 24, 2006; 46(2): 834 - 839. [Abstract] [Full Text] [PDF]

				D. R. Panthee, V. R. Pantalone, D. R. West, A. M. Saxton, and C. E. Sams Quantitative Trait Loci for Seed Protein and Oil Concentration, and Seed Size in Soybean Crop Sci., August 26, 2005; 45(5): 2015 - 2022. [Abstract] [Full Text] [PDF]