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CELL BIOLOGY & MOLECULAR GENETICS

Tetrasomic Linkage Mapping of RFLP, PCR, and Isozyme Loci in Lotus corniculatus L.

^a USDA-ARS, Rice Research Unit, 1509 Aggie Dr., Beaumont, TX 77713
^b National Forage Seed Production Res. Cntr., USDA-ARS, 3450 SW Campus Way, Corvallis, OR 97331
^c USDA-ARS, Plant Genetics Research Unit, Univ. Missouri, Columbia, MO 65211

^* Corresponding author (r-fjellstrom{at}tamu.edu)

Lotus corniculatus L. is a tetraploid (2n = 4x = 24) forage legume which has had limited inheritance studies. The purpose of this research was to develop a genetic map and characterize the genome of L. corniculatus using molecular markers. A linkage map was constructed for L. corniculatus using information from 217 markers mapped as single-dose restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), sequence tagged site (STS), and isozyme markers and five duplex RFLP markers. Formulas for calculating maximum likelihood estimates of recombination involving codominantly scored duplex loci in tetrasomic species from direct count data are made available. Six composite linkage groups were constructed from the combined data of four homologous linkage groups anchored by shared RFLP loci. Three of the composite groups (Groups 2, 3, and 4) were well defined, two groups (Groups 5 and 6) were poorly discriminated because of apparent marker duplication found between them, and one group (Group 1) was poorly defined because of an apparently high degree of differentiation between its homologous members. This first genetic map for L. corniculatus spans 572.1 centimorgans (cM) and serves to provide a foundation for the genetic analysis of complex traits and marker-aided selection.

Abbreviations: cM, centimorgan • GR, glutathione reductase • ISSR, inter-simple sequence repeat • PCR, polymerase chain reaction • RAPD, randomly amplified polymorphic DNA • RFLP, restriction fragment length polymorphism • SSR, simple sequence repeat • STS, sequence tagged site • TBE, tris-borate