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GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY

Physical and Genetic Mapping of Wheat Kinase Analogs and NBS-LRR Resistance Gene Analogs

^a Dep. of Plant Pathology, 4024 Throckmorton Hall, Kansas State University, Manhattan, KS 66506-5502
^b USDA-ARS Cereal Crops Research Unit, Northern Crop Science Laboratory, Fargo, ND 58105-5677
^c USDA-ARS Plant Science and Entomology Research Unit, Manhattan, KS 66506-5502

^* Corresponding author (jpf{at}alfalfa.ksu.edu)

Conserved motifs within resistance genes have been utilized in polymerase chain reaction (PCR)-based strategies to isolate resistance gene analogs (RGAs) and resistance gene-like (RGLs) sequences from many plant species. RGAs have the potential to serve as closely linked markers for marker-assisted breeding or even as resistance gene candidates. The objectives of this study were to clone, sequence, and map RGAs and kinase analogs (KA). Three motifs in nucleotide binding site-leucine-rich repeat (NBS-LRR) resistance genes and two conserved motifs within R-gene kinases were used to design degenerate primers and amplify RGAs and KAs from wheat (Triticum aestivum L.). Eight NBS-LRR and 26 KAs were isolated. The clones were physically mapped to chromosomes by means of wheat nulli-tetrasomic lines. The probes detected 137 fragments that could be assigned to 20 of the 21 chromosomes; nearly half of the fragments mapped in the B genome. None of the fragments mapped to chromosome 4D. Genetic mapping of clones showed simple and complex loci indicating both single and multigene families. The RGAs and KAs will be useful as markers for mapping resistance gene loci.

Abbreviations: KA, kinase analogs • ITMI, International Triticeae Mapping Initiative • NT, nullisomic-tetrasomic line • PCR, polymerase chain reaction • R-gene, resistance gene • RGA, resistance gene analog • RGL, resistance gene-like

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