HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (14) Citing Articles via Google Scholar Google Scholar Articles by Cober, E. R. Articles by Simmonds, D. H. Search for Related Content PubMed Articles by Cober, E. R. Articles by Simmonds, D. H. Agricola Articles by Cober, E. R. Articles by Simmonds, D. H. Related Collections Crop Genetics Soybean

CROP BREEDING, GENETICS & CYTOLOGY

Partial Resistance to White Mold in a Transgenic Soybean Line

^a Agriculture and Agri-Food Canada, ECORC, Building 110, Central Exp. Farm, Ottawa, ON, K1A 0C6 Canada
^b Centre de Recherche sur les Grains, Inc., 2700 rue Einstein, bur. D1 300.24A, Sainte-Foy, QC, G1P 3W8 Canada
^c Dep. of Plant Agriculture, Crop Sci. Bldg., Univ. of Guelph, Guelph, ON, N1G 2W1 Canada

* Corresponding author (coberer{at}em.agr.ca)

Oxalic acid is an important pathogenic factor for the fungus Sclerotinia sclerotiorum (Lib.) de Bary. An oxalate degrading enzyme, oxalate oxidase (OxO), in transgenic soybean [Glycine max (L.) Merr.] has reduced pathogen growth in indoor seedling studies. The objective of this study was to characterize the response of this OxO transgenic soybean line to white mold under field conditions and also to characterize the agronomic performance of this transgenic line under noninfected conditions. The transgenic line 80(30)-1contains the wheat germin gene gf-2.8 that codes for OxO. This line was compared with 80(30)-9, a sib line which does not contain the gene, as well as resistant and susceptible cultivars. Field tests were conducted at three sites infested with white mold in Ontario and Quebec across 3 yr. The Sainte-Foy site provided the highest infection potential and the transgenic line had a disease severity index (DSI) of 7 compared with 80(30)-9 with a DSI of 46, across 3 yr. Across 2 yr, resistant commercial cultivars had an average DSI of 2 and susceptible cultivars a DSI of 46. Stem inoculations were performed in the field at Elora and the transgenic line was significantly less infected compared with 80(30)-9. In noninfested trials, no significant differences were found between the transgenic, the negative sib, and the parental lines for seed yield, maturity, seed weight, or seed protein and oil content. The transgene provided white mold resistance equivalent to the best commercial cultivars in a white mold susceptible background.

Abbreviations: DSI, disease severity index • OxO, oxalate oxidase • PDA, potato dextrose agar • QTL, qualitative trait loci

This article has been cited by other articles:

				G. Zimmermann, H. Baumlein, H.-P. Mock, A. Himmelbach, and P. Schweizer The Multigene Family Encoding Germin-Like Proteins of Barley. Regulation and Function in Basal Host Resistance Plant Physiology, September 1, 2006; 142(1): 181 - 192. [Abstract] [Full Text] [PDF]

				R. A. Gilbert, M. Gallo-Meagher, J. C. Comstock, J. D. Miller, M. Jain, and A. Abouzid Agronomic Evaluation of Sugarcane Lines Transformed for Resistance to Sugarcane mosaic virus Strain E Crop Sci., August 26, 2005; 45(5): 2060 - 2067. [Abstract] [Full Text] [PDF]