HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free An erratum has been published Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Jia, Y. Articles by Singh, P. Search for Related Content PubMed Articles by Jia, Y. Articles by Singh, P. Agricola Articles by Jia, Y. Articles by Singh, P. Related Collections Cell Biology & Molecular Genetics Plant Disease Crop Genetics

CELL BIOLOGY & MOLECULAR GENETICS

Development of Dominant Rice Blast Pi-ta Resistance Gene Markers

^a USDA-ARS, Dale Bumpers National Rice Research Center, P. O. Box 1090, Stuttgart, AR 72160-0287
^b Institute of Nuclear Agricultural Sciences, Zhejiang Univ., Hangzhou, P. R. China 310029

* Corresponding author (yjia{at}spa.ars.usda.gov)

Incorporation of resistance genes into existing rice (Oryza sativa L.) cultivars is a powerful strategy and is commonly applied in breeding rice resistance to blast disease [caused by Pyricularia grisea Sacc. = P. oryzae Cavara (teleomorph: Magnaporthe grisea (Hebert) Barr)]. The rice blast resistance gene, Pi-ta, originally introgressed into japonica from indica rice is important in breeding for rice blast resistance worldwide. In the southern USA, the rice cultivar Katy contains Pi-ta and is resistant to the predominant blast M. grisea races IB-49 and IC-17 and has been used as the blast resistant breeding parent. Three pairs of DNA primers specific to the dominant indica Pi-ta gene were designed to amplify the Pi-ta DNA fragments by polymerase chain reaction (PCR). PCR products amplified by these Pi-ta specific primers were cloned and sequenced. Sequence analysis confirmed the presence of the dominant indica Pi-ta allele. These Pi-ta primers were used to examine the presence of Pi-ta alleles in advanced Arkansas rice breeding lines. The Pi-ta containing rice lines, as determined by PCR analysis, were resistant to both IB-49 and IC-17 in standard pathogenicity assays. In contrast, lines lacking the Pi-ta genes failed to protect rice plants against both races IB-49 and IC-17. The presence of Pi-ta markers correlated with the Pi-ta resistance spectrum. Thus, the Pi-ta gene markers provide a basis for stacking other blast resistance genes into high yielding and good quality advanced breeding rice lines.

This article has been cited by other articles:

				G. C. Eizenga, H. A. Agrama, F. N. Lee, W. Yan, and Y. Jia Identifying Novel Resistance Genes in Newly Introduced Blast Resistant Rice Germplasm Crop Sci., July 25, 2006; 46(5): 1870 - 1878. [Abstract] [Full Text] [PDF]

				R. Fjellstrom, C. A. Conaway-Bormans, A. M. McClung, M. A. Marchetti, A. R. Shank, and W. D. Park Development of DNA Markers Suitable for Marker Assisted Selection of Three Pi Genes Conferring Resistance to Multiple Pyricularia grisea Pathotypes Crop Sci., September 1, 2004; 44(5): 1790 - 1798. [Abstract] [Full Text] [PDF]