| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Biotechnology Research Unit, Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali, Colombia
b Long Ashton Research Station of the Institute of Arable Crops Research (IACR), Bristol, UK
* Corresponding author (j.tohme{at}cgiar.org)
Phaseolus beans are distributed worldwide and are cultivated in the tropics, subtropics, and temperate zones. The common bean is the most important grain legume for direct human consumption in the world. The objectives of this study were to isolate microsatellite repeats and to establish their discriminatory power (DL) to be used in bean diversity characterization and mapping. We isolated, cloned, and sequenced genomic DNA fragments that contained microsatellite loci from three genomic libraries of Phaseolus vulgaris L. The polymorphism of the microsatellites was evaluated in a panel of 21 P. vulgaris genotypes made up of cultivated and wild beans from the Mesoamerican and Andean pools, and nine genotypes from four Phaseolus species. The number of alleles per microsatellite locus ranged from 1 to 14, with an average of 6 alleles per primer pair. Almost all the microsatellite loci showed high levels of discriminatory power, with the highest value being 0.94. These results indicate that microsatellites can be valuable genetic markers for assessing genetic diversity in the P. vulgaris. The high levels of polymorphism of these new bean microsatellites and their wide cross-species transportability make these new markers useful for mapping and molecular characterization of Phaseolus species.
Abbreviations: SSR, simple sequence repeats • bp, base pairs • PCR, polymerase chain reaction • DL, Discriminatory power
This article has been cited by other articles:
|
|
 |
|
  E. Perez-Vega, A. Campa, L. De la Rosa, R. Giraldez, and J. J. Ferreira Genetic Diversity in a Core Collection Established from the Main Bean Genebank in Spain Crop Sci., June 26, 2009; 49(4): 1377 - 1386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kwak, J. A. Kami, and P. Gepts The Putative Mesoamerican Domestication Center of Phaseolus vulgaris Is Located in the Lerma-Santiago Basin of Mexico Crop Sci., March 17, 2009; 49(2): 554 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Cichy, M. W. Blair, C. H. Galeano Mendoza, S. S. Snapp, and J. D. Kelly QTL Analysis of Root Architecture Traits and Low Phosphorus Tolerance in an Andean Bean Population Crop Sci., January 28, 2009; 49(1): 59 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kaga, T. Isemura, N. Tomooka, and D. A. Vaughan The Genetics of Domestication of the Azuki Bean (Vigna angularis) Genetics, February 1, 2008; 178(2): 1013 - 1036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Acosta-Gallegos, J. D. Kelly, and P. Gepts Prebreeding in Common Bean and Use of Genetic Diversity from Wild Germplasm Crop Sci., December 18, 2007; 47(Supplement_3): S-44 - S-59. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Maxwell, M. A. Brick, P. F. Byrne, H. F. Schwartz, X. Shan, J. B. Ogg, and R. A. Hensen Quantitative Trait Loci Linked to White Mold Resistance in Common Bean Crop Sci., November 7, 2007; 47(6): 2285 - 2294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Gelin, S. Forster, K. F. Grafton, P. E. McClean, and G. A. Rojas-Cifuentes Analysis of Seed Zinc and Other Minerals in a Recombinant Inbred Population of Navy Bean (Phaseolus vulgaris L.) Crop Sci., July 30, 2007; 47(4): 1361 - 1366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Martinez-Castillo, D. Zizumbo-Villarreal, P. Gepts, and P. Colunga-GarciaMarin Gene Flow and Genetic Structure in the Wild-Weedy-Domesticated Complex of Phaseolus lunatus L. in its Mesoamerican Center of Domestication and Diversity Crop Sci., January 22, 2007; 47(1): 58 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. E. Ochoa, M. W. Blair, and J. P. Lynch QTL Analysis of Adventitious Root Formation in Common Bean under Contrasting Phosphorus Availability Crop Sci., May 18, 2006; 46(4): 1609 - 1621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Martinez-Castillo, D. Zizumbo-Villarreal, P. Gepts, P. Delgado-Valerio, and P. Colunga-GarciaMarin Structure and Genetic Diversity of Wild Populations of Lima Bean (Phaseolus lunatus L.) from the Yucatan Peninsula, Mexico Crop Sci., March 27, 2006; 46(3): 1071 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Mason, M. R. Stevens, E. N. Jellen, A. Bonifacio, D. J. Fairbanks, C. E. Coleman, R. R. McCarty, A. G. Rasmussen, and P. J. Maughan Development and Use of Microsatellite Markers for Germplasm Characterization in Quinoa (Chenopodium quinoa Willd.) Crop Sci., June 24, 2005; 45(4): 1618 - 1630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Frei, M. W. Blair, C. Cardona, S. E. Beebe, H. Gu, and S. Dorn QTL Mapping of Resistance to Thrips palmi Karny in Common Bean Crop Sci., January 1, 2005; 45(1): 379 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. J. Gomez, M. W. Blair, B. E. Frankow-Lindberg, and U. Gullberg Molecular and Phenotypic Diversity of Common Bean Landraces from Nicaragua Crop Sci., July 1, 2004; 44(4): 1412 - 1418. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| The SCI Journals | Agronomy Journal | Vadose Zone Journal | |||
| Journal of Natural Resources and Life Sciences Education |
Soil Science Society of America Journal | ||||
| Journal of Plant Registrations | Journal of Environmental Quality |
The Plant Genome | |||
