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CELL BIOLOGY & MOLECULAR GENETICS

Mapping and Progress toward Map-Based Cloning of Brown Planthopper Biotype-4 Resistance Gene Introgressed from Oryza officinalis into Cultivated Rice, O. sativa

^a Dep. of Soil and Crop Sciences, Texas A&M Univ., College Station, TX 77843
^b Dep. of Agronomy, Kansas State Univ., Manhattan, KS 66506
^c Dow Agro Sciences, 9330 Zionsville Road, Indianapolis, IN 46268-1054
^d Dep. of Plant Breeding and Biometry, Cornell Univ., Ithaca, NY 14853-1901
^e Centre for Plant Molecular Biology, Tamil Nadu Agricultural Univ., Coimbatore 641 003, India

* Corresponding author (renga{at}tamu.edu)

Brown plant hopper (BPH), Nilaparvata lugens (Stal.), is a serious insect pest of rice in Asia, causing direct losses and vectoring Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV). Recombinant inbred lines (RILs) developed from a cross between ‘IR50’ and ‘IR54745-2-21-12-17-6’ were used to identify random amplified polymorphic DNA (RAPD) markers closely linked to a BPH Biotype-4 resistance gene [Bph13 (t)] derived from Oryza officinalis Wall. Bulked segregant analysis (BSA) using RAPD primers identified 11 polymorphic fragments. Six fragments, AJ09₂₆₀a, AL05₂₂₀a, AK10₆₉₀a, AK10₄₃₀c, AK10₃₈₀d, and AJ01₂₀₀a, were linked in coupling phase to the Bph13 (t) locus. The remaining five fragments, AJ09₂₃₀b, AJ09₁₈₀c, AJ09₁₀₀d, AL05₄₀₀b, and AK10₃₄₀e, were linked in repulsion. The most closely linked RAPD marker, AJ09₂₃₀b, was converted to a codominant linked sequence tagged sites (STS) marker. This marker mapped 1.3 centimorgans (cM) from the resistance gene and was placed on rice chromosome 3 by means of ‘IR64’ x ‘Azucena’ doubled haploid (DH) population. The tightly linked STS marker could be used for marker-assisted selection (MAS). In addition, these markers will be useful for a positional cloning strategy to isolate the resistance gene.

Abbreviations: BPH, brown planthopper • bp, base pairs • BSA, bulked segregant analysis • cM, centimorgans • DH, doubled haploid • MAS, marker-assisted selection • QTL, quantiative trait locus • RILs, recombinant inbred lines • RAPD, random amplified polymorphic DNA • STS, sequence tagged sites

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