HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Liu, Z. Articles by Kaeser, O. Search for Related Content PubMed Articles by Liu, Z. Articles by Kaeser, O. Agricola Articles by Liu, Z. Articles by Kaeser, O. Related Collections Cell Biology & Molecular Genetics

CELL BIOLOGY & MOLECULAR GENETICS

A PCR Assay for Rapid Discrimination of Sterile Cytoplasm Types in Maize

Institute of Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland

* Corresponding author (peter.stamp{at}ipw.agrl.ethz.ch)

A single-seed, multiplex polymerase chain reaction (PCR) protocol is presented to distinguish the three major types of cytoplasmic male sterility (CMS-T, -C, and -S) in maize (Zea mays L.). Three pairs of PCR primers were designed, corresponding to the chimeric regions of mitochondrial DNA sequences specific for the three types of CMS cytoplasm. The specificity of these primers was tested by means of the inbred line B37 with male-sterile (T, C, and S) and normal (N) cytoplasm as reference material. Subsequently, a single-seed PCR assay was developed for the determination of cytoplasm types in a single reaction and applied to a wide range of inbred lines with normal and sterile cytoplasm (T, C, and S) and with predominantly identified subtypes of the major sterile cytoplasm types. Our protocol proved to be a rapid, inexpensive, and reliable procedure for the molecular classification of male sterile maize cytoplasm to the three major categories T, C, and S.

Abbreviations: CMS, cytoplasmic male sterility • mtDNA, mitochondrial DNA • PCR, polymerase chain reaction • RFLP, restriction fragment length polymorphism

This article has been cited by other articles:

				U. Weingartner, O. Kaeser, M. Long, and P. Stamp Combining Cytoplasmic Male Sterility and Xenia Increases Grain Yield of Maize Hybrids Crop Sci., November 1, 2002; 42(6): 1848 - 1856. [Abstract] [Full Text] [PDF]