HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (16) Citing Articles via Google Scholar Google Scholar Articles by Jeong, S. C. Articles by Maroof, M. A. S. Search for Related Content PubMed PubMed Citation Articles by Jeong, S. C. Articles by Maroof, M. A. S. Agricola Articles by Jeong, S. C. Articles by Maroof, M. A. S. Related Collections Cell Biology & Molecular Genetics

CELL BIOLOGY & MOLECULAR GENETICS

Genetic and Sequence Analysis of Markers Tightly Linked to the Soybean mosaic virus Resistance Gene, Rsv3

^a Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA 24061-0404
^b Monsanto Company, 3302 SE Convenience Blvd., Ankeny, IA 50021
^c USDA-ARS Small Fruits Research Unit, 306 S. High St., Poplarville, MS 39470

* Corresponding author (smaroof{at}vt.edu)

Soybean mosaic virus (SMV) is a major viral pathogen, affecting soybean [Glycine max (L.) Merr.] production worldwide. The Rsv3 gene of soybean confers resistance to three of the most virulent strains (G5–G7) of SMV. The objectives of this study were to map Rsv3 and develop polymerase chain reaction (PCR) based markers for marker-assisted selection (MAS) purposes. Disease-response data were collected from two F₂ mapping populations, L29 (Rsv3) x Lee68 (rsv3) and Tousan 140 (Rsv3) x Lee68 (rsv3). Bulk segregant analysis based on amplified fragment length polymorphism (AFLP) markers demonstrated that the Rsv3 locus maps to the soybean molecular linkage group (MLG) B2 between restriction fragment length polymorphism (RFLP) markers A519 and Mng247. These two tightly linked RFLP markers were converted to PCR-based markers to expedite MAS. Sequence analysis of the Mng247 genomic region revealed similarity to the consensus sequence of a leucine-rich repeat (LRR) characteristic of the extracellular LRR class of disease resistance genes. Results from this study will be useful in pyramiding viral resistance genes and in cloning the Rsv3 gene.

Abbreviations: AFLP, amplified fragment length polymorphism • bp, base pairs • cM, centimorgan • LRR, leucine-rich repeat • MAS, marker-assisted selection • MLG, molecular linkage group • NIL, near isogenic line • PCR, polymerase chain reaction • RFLP, restriction fragment length polymorphism • SMV, Soybean mosaic virus

This article has been cited by other articles:

				A. Shi, P. Chen, D. X. Li, C. Zheng, A. Hou, and B. Zhang Genetic Confirmation of 2 Independent Genes for Resistance to Soybean Mosaic Virus in J05 Soybean Using SSR Markers J. Hered., May 19, 2008; (2008) esn035v1. [Abstract] [Full Text] [PDF]

				M. A. Saghai Maroof, S. C. Jeong, I. Gunduz, D. M. Tucker, G. R. Buss, and S. A. Tolin Pyramiding of Soybean Mosaic Virus Resistance Genes by Marker-Assisted Selection Crop Sci., March 19, 2008; 48(2): 517 - 526. [Abstract] [Full Text] [PDF]

				A. Shi, P. Chen, C. Zheng, A. Hou, and B. Zhang A PCR-based Marker for the Rsv1 Locus Conferring Resistance to Soybean Mosaic Virus Crop Sci., January 16, 2008; 48(1): 262 - 268. [Abstract] [Full Text] [PDF]

				K. Yang and S.-C. Jeong Genetic Linkage Map of the Nucleolus Organizer Region in the Soybean Genetics, January 1, 2008; 178(1): 605 - 608. [Abstract] [Full Text] [PDF]

				G. Ma, P. Chen, G. R. Buss, and S. A. Tolin Genetics of Resistance to Two Strains of Soybean Mosaic Virus in Differential Soybean Genotypes J. Hered., July 1, 2004; 95(4): 322 - 326. [Abstract] [Full Text] [PDF]