HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Sharp, G. L. Articles by Talbert, L. E. Search for Related Content PubMed PubMed Citation Articles by Sharp, G. L. Articles by Talbert, L. E. Agricola Articles by Sharp, G. L. Articles by Talbert, L. E. Related Collections Germplasm Enhancement Cell Biology & Molecular Genetics Crop Genetics

CROP BREEDING, GENETICS & CYTOLOGY

Field Evaluation of Transgenic and Classical Sources of Wheat streak mosaic virus Resistance

Dep. of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717

* Corresponding author (usslt{at}montana.edu)

The development of wheat (Triticum aestivum L.) cultivars that are resistant to Wheat streak mosaic virus (WSMV), yet competitive in yield under nondiseased conditions, is an objective for breeding programs in the Great Plains. This field study was conducted to compare classical and transgenic sources of resistance to WSMV. Three sets of germplasm were evaluated. These included adapted cultivars with various levels of tolerance, transgenic wheat lines containing viral coat protein or replicase sequences from WSMV that showed resistance in greenhouse trials, and germplasm with resistance to WSMV due to a translocated segment of chromosome 4Ai-2 from Thinopyrum intermedium (Host) Barkworth and Dewey containing Wsm1. A replicated field trial was conducted at Bozeman, MT, over a two-year period to evaluate the effectiveness of these different sources of resistance to mechanical inoculation of WSMV. Adapted cultivars differed in their ability to tolerate WSMV with mean reductions in yield over the two years ranging from 41 to 74%. Incorporation of the replicase or coat protein gene from WSMV did not provide field resistance to viral infection and in general, transgenic lines yielded less than their parent cultivar, ‘Hi-Line’. Wheat-Thinopyrum lines positive for a DNA marker linked to the Wsm1 gene had significantly reduced yield losses ranging from 5 to 39% compared with yield losses of 57 to 88% in near isogenic lines not having the Wsm1 gene. Yield of lines with Wsm1 in the absence of disease ranged from 11 to 28% less than yield of lines without Wsm1. Our results suggest Wsm1 provides the best source of WSMV resistance but a yield penalty may exist because of the presence of the translocation.

This article has been cited by other articles:

				F. D. Meyer, L. E. Talbert, J. M. Martin, S. P. Lanning, T. W. Greene, and M. J. Giroux Field Evaluation of Transgenic Wheat Expressing a Modified ADP-Glucose Pyrophosphorylase Large Subunit Crop Sci., February 6, 2007; 47(1): 336 - 342. [Abstract] [Full Text] [PDF]

				A. Anand, T. Zhou, H. N. Trick, B. S. Gill, W. W. Bockus, and S. Muthukrishnan Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum J. Exp. Bot., March 1, 2003; 54(384): 1101 - 1111. [Abstract] [Full Text] [PDF]