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CELL BIOLOGY & MOLECULAR GENETICS

A Retrospective DNA Marker Assessment of the Development of Insect Resistant Soybean

^a Monsanto Company, Galena, MD 21635
^b Dep. of Crop and Soil Sciences, Univ. of Georgia, Athens, GA 30602-7272
^c Dep. of Entomology, Univ. of Georgia, Athens, GA 30602-7272
^d USDA/ARS, Crop Genetics and Breeding Research Unit, Tifton, GA 31793

* Corresponding author (rboerma{at}arches.uga.edu)

There has been limited success over the past 30 yr in the development of superior soybean cultivars [Glycine max (L.) Merr] with insect resistance. Success may be hampered by the quantitative nature of resistance and by linkage drag from resistant plant introduction (PI) donor parents. Soybean insect resistance quantitative trait loci (SIR QTLs) have been identified from PI 229358 and PI 171451 by restriction fragment length polymorphism (RFLP) analysis. The objective of this study was to tag the SIR QTLs from PI 229358 with simple sequence repeat (SSR) markers and to determine the extent to which the SIR QTLs have been introgressed in registered cultivars, germplasm releases, or breeding lines that have resistance derived from this PI or from PI 171451. Marker analysis defined intervals by 5 centimorgans (cM) or less for a SIR QTL on linkage group D1b (SIR-D1b), and for SIR-G, SIR-H, and SIR-M. SIR QTLs were tracked through pedigrees by evaluating the inheritance of PI alleles at marker loci tightly linked to the QTLs during the phenotypic selection for insect resistance. It was inferred that at least 13 of the 15 SIR genotypes studied had introgressed SIR-M. PI genome introgression around SIR-M was measured to assess linkage drag. Some genotypes exhibited a dramatic reduction in the amount of linked PI genome, which likely occurred in response to phenotypic selection for agronomic performance as a means of reducing linkage drag. Only a few genotypes were inferred to possess SIR-G or SIR-H, and no genotypes possessed SIR-D1b. The results of this study indicate that marker-assisted selection for SIR QTLs is needed to introgress these loci into elite genetic backgrounds.

Abbreviations: CEW, corn earworm • cM, centimorgan • LG, linkage group • MAS, marker-assisted selection • MBB, Mexican bean beetle • PI, plant introduction • QTL, quantitative trait loci • RFLP, restriction fragment length polymorphism • SSR, simple sequence repeat • SIR, soybean insect resistance • SBL, soybean looper • VBC, velvetbean caterpillar

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