HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (14) Citing Articles via Google Scholar Google Scholar Articles by Lewis, L. C. Articles by Bidne, K. G. Search for Related Content PubMed Articles by Lewis, L. C. Articles by Bidne, K. G. Agricola Articles by Lewis, L. C. Articles by Bidne, K. G. Related Collections Crop Genetics Maize Plant Disease

CROP BREEDING, GENETICS & CYTOLOGY

Assessment of Plant Pathogenicity of Endophytic Beauveria bassiana in Bt Transgenic and Non-Transgenic Corn

USDA-ARS, Corn Insects & Crop Genetics Research Unit, Ames, Iowa 50011

* Corresponding author (leslewis{at}iastate.edu)

Field and greenhouse studies were conducted to determine the proclivity of Bacillus thuringiensis (Bt) (Berliner)-transgenic corn (Zea mays L.) to form an endophytic relationship with Beauveria bassiana, and to evaluate the corn for possible plant pathological effects associated with this relationship. Beauveria bassiana (Balsamo) Vuillemin was applied as a granular formulation to two separate lines of corn, expressing Bt events MON802 and MON810, and their corresponding isolines. There were no significant differences in levels of endophytism between transgenic events or their near-isolines. In greenhouse studies, B. bassiana was applied as a liquid seed treatment to Bt transgenic corn hybrids Pioneer 34R06 (event MON810) and Ciba Max 454 (event 176) and their near isolines at a rate of 2 x 10¹⁰ conidia per ml. There were no significant differences in seed germination or presence of root pathogens in transgenic or isoline seeds soaked in a B. bassiana suspension. The same lines of corn were used in field experiments with treatments of seeds soaked in a suspension of B. bassiana, a foliar application of a granular formulation of B. bassiana, and corresponding untreated checks. Plants were sampled throughout the growing season and evaluated for growth of individual plant components, including sheaths, leaves, stem, husk, ear, plant leaf-to-stem ratio and overall plant growth. There were no significant differences in overall plant growth between the B. bassiana treatments or in the growth of each plant component. The results of this study indicate that B. bassiana readily forms an endophytic relationship with transgenic and non-transgenic corn and causes no plant pathology.

Abbreviations: GLM, General linear models • CFU, colony forming units • ARSEF, ARS collection of entomopathogenic fungi

This article has been cited by other articles:

				J. Wu, H. J. Ridgway, M. A. Carpenter, and T. R. Glare Identification of novel genes associated with conidiation in Beauveria bassiana with suppression subtractive hybridization Mycologia, January 1, 2008; 100(1): 20 - 30. [Abstract] [Full Text] [PDF]

				E.-M. Cho, L. Liu, W. Farmerie, and N. O. Keyhani EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. I. Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia. Microbiology, September 1, 2006; 152(Pt 9): 2843 - 2854. [Abstract] [Full Text] [PDF]

				E.-M. Cho, D. Boucias, and N. O. Keyhani EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. II. Fungal cells sporulating on chitin and producing oosporein. Microbiology, September 1, 2006; 152(Pt 9): 2855 - 2864. [Abstract] [Full Text] [PDF]