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CROP BREEDING, GENETICS & CYTOLOGY

An Isozyme Marker for Resistance to Root-Knot Nematode in Sugarbeet

^a USDA-ARS-PWA, U.S. Agric. Res. Stn., 1636 East Alisal St., Salinas, CA 93905-3018
^b Harris Moran Seed Co., 100 Breen Rd., San Juan Bautista, CA 95045

* Corresponding author (myu{at}salinas.ars.usda.gov)

Root-knot nematode (Meloidogyne spp.) is a destructive pest of sugarbeet (Beta vulgaris L.) that reduces production in infested areas and is difficult to manage. Identification of nematode-resistant plants is a time-consuming process that is subject to genotype–environment interaction. Development of resistant cultivars/hybrids is the most effective control. This study was conducted to establish a rapid and effective screening technique to detect a large number of sugarbeet genotypes with resistance to Meloidogyne spp. A nematode-resistant sugarbeet germplasm line, Mi-1 Beta, was previously developed using J2 inoculation and screening procedures. Leaf and cotyledon extractions were used in diagnosis. Phosphoglucomutase (PGM) was found to be a potentially useful isozyme marker of resistance in Mi-1 Beta and derived lines in starch gel electrophoresis. Seven banding patterns (four resistant and three susceptible) were produced. All susceptible plants shared the banding pattern of the resistant strains, except for a single PGM band. If demonstrated to be tightly linked to nematode resistance, this novel PGM isozyme marker will accelerate breeding sugarbeet with resistance to root-knot nematode.

Abbreviations: PGM, phosphoglucomutase • PMS, phenazine methosulfate

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