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CELL BIOLOGY & MOLECULAR GENETICS

Partial Resistance of Transgenic Peas to Alfalfa Mosaic Virus under Greenhouse and Field Conditions

^a New Zealand Institute for Crop & Food Research Ltd, PO Box 4704, Christchurch, New Zealand
^b New Zealand Plant Breeding Ltd, PO Box 19, Lincoln, New Zealand

* Corresponding author (timmermang{at}crop.cri.nz)

Transformed pea (Pisum sativum L.) lines were produced with two chimeric gene constructs encoding the coat protein (CP) of alfalfa mosaic virus (AMV) strain NZ1. To determine whether transformed lines have improved AMV resistance, progeny of independently derived transgenic lines were tested in the greenhouse and five lines with improved virus resistance were identified. Resistance was observed only in individual plants that accumulated detectable CP product from the transgene, suggesting that resistance is CP mediated. Plants that accumulated detectable amounts of transgene CP product yet were susceptible to AMV were found among the progeny of most lines, indicating that these lines are only partially resistant to the challenging AMV strains, 425 and NZ1(Lincoln). A field test was conducted with the progeny of four independently derived transgenic lines. To test for improved virus resistance under field conditions, plots of transgenic lines and nontransgenic controls were inoculated with two AMV strains, NZ1(Lincoln) and NZ34. Analysis of disease severity in seedlings in inoculated plots confirmed that partial virus resistance can be produced by genetically modifying peas with AMV CP sequences.

Abbreviations: AMV, alfalfa mosaic virus • CP, coat protein • PCR, polymerase chain reaction • RT, reverse transcription • T₀, T₁, T₂ and T₃, independently-derived transgenic lines and first, second and third generation inbred progeny, respectively • T-DNA, transfer DNA • TMV, tobacco mosaic virus