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a Laboratorio de Marcadores Moleculares, Instituto de Recursos Biológicos CIRN-INTA, 1712 Castelar, Buenos Aires, Argentina
b Instituto de Biotecnología CICVyA-CNIA, INTA 1712 Castelar, Buenos Aires, Argentina
c Dep. of Agronomy and Range Science, Univ. of California, Davis, CA 95616
* Corresponding author (jdubcovsky{at}ucdavis.edu)
Characterization of germplasm by means of DNA fingerprinting techniques provides a tool for precise germplasm identification and a quantitative estimate of genetic diversity. This estimate is important because a decrease in genetic variability might result in a reduction of the plasticity of the crops to respond to changes in climate, pathogen populations, or agricultural practices. In this study, 105 Argentine bread wheat (Triticum aestivum L.) cultivars released between 1932 and 1995 were characterized by simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. A selected subset of 10 highly informative SSR was used to construct an Identification Matrix that allowed the discrimination of the 105 cultivars. Data obtained from SSR markers were complemented by information derived from AFLPs. Molecular data were used to quantify genetic diversity across Argentine wheat breeding programs and to determine if modern wheat cultivars have a lower genetic diversity than earlier cultivars (genetic erosion). No significant differences in genetic diversity were found among the large private and public breeding programs, suggesting that each of them contains a representative sample of the complete diversity of the Argentine germplasm. Significant differences were found for both SSR and AFLP only between breeding programs with large differences in number of released cultivars. No significant differences in genetic diversity were found between the group of cultivars released before 1960 and those released in each of the following three decades. Average diversity values based on SSR markers were almost identical for the four analyzed periods. Genetic diversity estimates based on AFLP data confirmed the absence of a reduction of genetic diversity with time, but significant differences (P = 0.01) were found between bread wheat cultivars released in the 1970s (PIC = 0.28) and those released in the 1980s (PIC = 0.34). These results show that the Argentine bread wheat germplasm has maintained a relatively constant level of genetic diversity during the last half century.
Abbreviations: AFLP, amplified fragment length polymorphism • bp, base pairs • GS, genetic similarity • f, kinship coefficient • PIC, Polymorphism Index Content • r, correlation coefficient • RFLP, restriction fragment length polymorphism • SSR, simple sequence repeat or microsatellite • UPGMA, unweighted pair-group method with arithmetic averages
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