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CELL BIOLOGY & MOLECULAR GENETICS

Molecular Markers Linked to Brown Stem Rot Resistance Genes, Rbs₁ and Rbs₂, in Soybean

^a Syngenta Seeds, Inc., 317-330th St., Stanton, MN 55018
^b Monsanto, 634 East Lincoln Way, Ames, IA 50010
^c Dep. of Crop Sciences, Univ. of Illinois, 1102 S. Goodwin Ave., Urbana, IL 61801
^d College of Agricultural and Life Sciences, UW-Madison, 1630 Linden Dr., Madison, WI 53706

Corresponding author (mike.bachman{at}syngenta.com)

Brown stem rot (BSR) of soybean [Glycine max (L.) Merr.] is caused by the fungal pathogen Phialophora gregata (Allington & D.W. Chamberlain) W. Gams and occurs in soybean production areas around the world. Brown stem rot resistance genes Rbs₁, Rbs₂, and Rbs₃ have been identified in soybean germplasm and plant introductions through traditional genetic analyses. Resistance to BSR has been shown to reduce yield losses in soybean, but selection for this trait is laborious and confounded by environmental variation. The objectives of this study were to identify molecular markers linked to BSR resistance genes Rbs₁ and Rbs₂, and map these genes in the soybean genome. Genetic families of populations segregating for Rbs₁ and Rbs₂ were evaluated in the greenhouse for BSR phenotypic reaction and identified as resistant, segregating, or susceptible. Leaf tissue collected from members of F_2:3 families was bulked and DNA simple sequence repeat (SSR) marker analysis was used to identify markers that cosegregated with BSR reaction phenotypes. Five pairs of Rbs₂ near-isogenic lines were subjected to a similar analysis to verify results obtained from marker analysis conducted on the population segregating for Rbs₂. Results of marker analyses indicated that SSR markers Satt215 and Satt431 were linked to Rbs₁ and that Satt244 and Satt431 were linked to Rbs₂. Marker-assisted selection in the Rbs₁ (using Satt431) and Rbs₂ (using Satt244) populations would have correctly predicted 88 and 82%, respectively, of the BSR reaction phenotypes. The Rbs₁ and Rbs₂ loci map to Molecular Linkage Group J and lie in a region known to contain Rbs₃. This region also contains loci conditioning resistance to taxonomically diverse fungal pathogens and a locus affecting nodulation in response to a bacterial symbiont.

Abbreviations: AFLP, amplified fragment length polymorphism • BSR, brown stem rot • MLG, molecular linkage group • PCR, polymerase chain reaction • PI, plant introduction • QTL, quantitative trait locus/loci • RFLP, restriction fragment length polymorphism • SSR, simple sequence repeat

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