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NOTES

A more rapid method of total sugar analysis for soybean seed

^a Dep. of Agronomy, Iowa State Univ., Ames, IA 50011
^b Dep. of Food Science and Human Nutrition, Iowa State Univ., Ames, IA 50011
^c Dep. of Biochemistry, Biophysics, and Molecular Biology, Iowa State Univ., Ames, IA 50011

Corresponding author (wfehr{at}iastate.edu)

The total sugar content of soybean [Glycine max (L.) Merr.] cultivars is important to the soyfood industry. The method of total sugar analysis reported in the literature is labor intensive and time consuming. The objective of this study was to develop a more rapid method for the determination of total sugar in soybean seed. The original method was modified by reducing the amounts of reagents used, eliminating sample filtering, reducing the number of sample dilutions, and increasing the rate of acid hydrolysis. There was a significant difference between the original (187 g kg^-1) and the rapid (224 g kg^-1) method for the means of 10 samples with a broad range of total sugar, but no significant sample x method interaction was observed. The phenotypic correlation between the two methods for total sugar of the 10 samples was 0.91 (P < 0.05). The number of samples that could be analyzed in 1 d increased from 12 for the original method to 70 for the rapid method, and the estimated cost per sample was reduced from $5.34 to $0.92. The rapid method can be used to determine the relative differences among soybean samples for total sugar content.