HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Pilet, M.L. Articles by Delourme, R. Search for Related Content PubMed Articles by Pilet, M.L. Articles by Delourme, R. Agricola Articles by Pilet, M.L. Articles by Delourme, R. Related Collections Other Oil Crops Cell Biology & Molecular Genetics Plant Disease Crop Genetics

CELL BIOLOGY & MOLECULAR GENETICS

Stability of QTL for Field Resistance to Blackleg across Two Genetic Backgrounds in Oilseed Rape

UMR INRA-ENSAR, Amélioration des Plantes et Biotechnologies Végétales, BP 35327, 35653 Le Rheu Cedex, France

Corresponding author (rdelourm{at}rennes.inra.fr)

Blackleg, caused by Leptosphaeria maculans (Desm.) Ces. et de Not., is a major disease of oilseed rape (Brassica napus L.) worldwide. Molecular markers would be useful tools to assist breeding for blackleg resistance. The objective of this study was (i) to map and characterize quantitative trait loci (QTL) for field blackleg resistance in doubled haploid (DH) and F_2:3 populations from the cross `Darmor' (resistant) x `Samourai' (susceptible) and (ii) to compare QTL with those previously identified in the cross `Darmor-bzh' x `Yudal'. A total of 134 DH lines and 185 F_2:3 families were genotyped with random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers and assessed for a disease index of resistance in 1998 and/or 1999 in one location. Genetic maps derived from the two populations included a total of 257 and 81 markers, respectively. Up to 30% of these markers were common to the Darmor-bzh x Yudal map previously used. A total of six and four genomic regions were associated with resistance in the DH and F_2:3 populations, respectively. They collectively explained 36 to 42% of the variation within each year and population. Three of them were consistent across the two populations derived from Darmor x Samourai cross and expressed dominant or overdominant effects. Four favorable alleles were derived from the susceptible parent. A total of 16 genomic regions were revealed for blackleg resistance in the two crosses Darmor-bzh x Yudal and Darmor x Samourai studied. Four of them were consistent over the two crosses. The inconsistencies observed between populations and crosses can be explained by different genetic backgrounds and disease infestation levels. For marker-assisted selection, these results suggest that QTL mapping must be carried out separately for each population.

Abbreviations: DH, doubled haploid • MAS, marker-assisted selection • PCR, polymerase chain reaction • QTL, quantitative trait locus(i) • RAPD, random amplified polymorphic DNA • RFLP, restriction fragment length polymorphism

This article has been cited by other articles:

				L. Gout, M. Eckert, T. Rouxel, and M.-H. Balesdent Genetic Variability and Distribution of Mating Type Alleles in Field Populations of Leptosphaeria maculans from France Appl. Envir. Microbiol., January 1, 2006; 72(1): 185 - 191. [Abstract] [Full Text] [PDF]

				T. R. Stefaniak, D. L. Hyten, V. R. Pantalone, A. Klarer, and T. W. Pfeiffer Soybean Cultivars Resulted from More Recombination Events Than Unselected Lines in the Same Population Crop Sci., December 2, 2005; 46(1): 43 - 51. [Abstract] [Full Text] [PDF]

				R. Mihaljevic, H. F. Utz, and A. E. Melchinger Congruency of Quantitative Trait Loci Detected for Agronomic Traits in Testcrosses of Five Populations of European Maize Crop Sci., January 1, 2004; 44(1): 114 - 124. [Abstract] [Full Text] [PDF]