Crop Science Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Figures Only
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hayes, A. J.
Right arrow Articles by Maroof, M.A.S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hayes, A. J.
Right arrow Articles by Maroof, M.A.S.
Agricola
Right arrow Articles by Hayes, A. J.
Right arrow Articles by Maroof, M.A.S.
Crop Science 40:1434-1437 (2000)
© 2000 Crop Science Society of America

CELL BIOLOGY & MOLECULAR GENETICS

Molecular Marker Mapping of RSV4, a Gene Conferring Resistance to all Known Strains of Soybean Mosaic Virus

Alec J. Hayesa, Guorong Maa,b, Glenn R. Bussa and M.A.Saghai Maroofa

a Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA 24061-0404 USA
b Medtronic Sofamor Danek, 1800 Pyramid Place, Memphis, TN 38132 USA

smaroof{at}vt.edu

Soybean mosaic virus (SMV) is a prevalent viral pathogen of soybean [Glycine max (L.) Merr.] wherever it is grown. Several genes that confer resistance in soybean to soybean mosaic virus have been identified. One of these resistance loci, Rsv4, confers resistance to all the known strain groups of SMV. This study was conducted to determine the map position of the Rsv4 locus in the soybean genome. A population of 255 F2 individuals from the cross of the SMV resistant line LR2 (Rsv4) by the susceptible line Lee68 (rsv4) was evaluated in a mapping study. DNA from 12 to 15 F2 individuals being either homozygous resistant or susceptible were pooled to produce bulk resistant and bulk susceptible DNA samples. Parents and bulks were screened with 101 AFLP primer pairs and two linked polymorphisms were identified. A putative linked marker, amplified by the primers Mse-AAA and Eco-AAG, was converted to a restriction fragment length polymorphism (RFLP) clone and a single polymorphic band was mapped 4.8 cM away from Rsv4. The same band was mapped in a second population containing 400 mapped loci and it was determined that this marker was located on the soybean D1b linkage group. Subsequent mapping in the original Rsv4 mapping population showed that the Rsv4 locus is flanked by the microsatellite markers, Satt542 at 4.7 cM and Satt558 at 7.8 cM.

Abbreviations: AFLP, amplified fragment length polymorphism • bp, base pair • BSA, bulk segregant analysis • cM, centimorgan • LL, population LR2 x Lee68 • VP, population V71-370 x PI407162 • R, resistant • RFLP, restriction fragment length polymorphism • S, susceptible • SMV, soybean mosaic virus




This article has been cited by other articles:


Home page
 Crop Sci.Home page
D. M. Tucker, M. A. S. Maroof, S. C. Jeong, G. R. Buss, and S. A. Tolin
Validation and Interaction of the Soybean Mosaic Virus Lethal Necrosis Allele, Rsv1-n, in PI 507389
Crop Sci., June 26, 2009; 49(4): 1277 - 1283.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
A. Shi, P. Chen, D. X. Li, C. Zheng, A. Hou, and B. Zhang
Genetic Confirmation of 2 Independent Genes for Resistance to Soybean Mosaic Virus in J05 Soybean Using SSR Markers
J. Hered., November 1, 2008; 99(6): 598 - 603.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
M. A. Saghai Maroof, S. C. Jeong, I. Gunduz, D. M. Tucker, G. R. Buss, and S. A. Tolin
Pyramiding of Soybean Mosaic Virus Resistance Genes by Marker-Assisted Selection
Crop Sci., March 19, 2008; 48(2): 517 - 526.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
A. Shi, P. Chen, C. Zheng, A. Hou, and B. Zhang
A PCR-based Marker for the Rsv1 Locus Conferring Resistance to Soybean Mosaic Virus
Crop Sci., January 16, 2008; 48(1): 262 - 268.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
Y. Wang, H. A. Hobbs, C. B. Hill, L. L. Domier, G. L. Hartman, and R. L. Nelson
Evaluation of Ancestral Lines of U.S. Soybean Cultivars for Resistance to Four Soybean Viruses
Crop Sci., February 23, 2005; 45(2): 639 - 644.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
G. Ma, P. Chen, G. R. Buss, and S. A. Tolin
Genetics of Resistance to Two Strains of Soybean Mosaic Virus in Differential Soybean Genotypes
J. Hered., July 1, 2004; 95(4): 322 - 326.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
L. Liao, P. Chen, G. R. Buss, Q. Yang, and S. A. Tolin
Inheritance and Allelism of Resistance to Soybean Mosaic Virus in Zao18 Soybean From China
J. Hered., November 1, 2002; 93(6): 447 - 452.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
P. Chen, G. R. Buss, S. A. Tolin, I. Gunduz, and M. Cicek
A Valuable Gene in Suweon 97 Soybean for Resistance to Soybean Mosaic Virus
Crop Sci., March 1, 2002; 42(2): 333 - 337.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
S. C. Jeong, S. Kristipati, A. J. Hayes, P. J. Maughan, S. L. Noffsinger, I. Gunduz, G. R. Buss, and M. A. S. Maroof
Genetic and Sequence Analysis of Markers Tightly Linked to the Soybean mosaic virus Resistance Gene, Rsv3
Crop Sci., January 1, 2002; 42(1): 265 - 270.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
The SCI Journals Agronomy Journal Vadose Zone Journal
Journal of Natural Resources
and Life Sciences Education		 Soil Science Society of America Journal
Journal of Plant Registrations Journal of
Environmental Quality		 The Plant Genome
Copyright © 2000 by the Crop Science Society of America.