HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Igartua, E. Articles by Mather, D.E. Search for Related Content PubMed Articles by Igartua, E. Articles by Mather, D.E. Agricola Articles by Igartua, E. Articles by Mather, D.E.

CELL BIOLOGY & MOLECULAR GENETICS

Marker-Based Selection of QTL Affecting Grain and Malt Quality in Two-Row Barley

^a Dep. of Plant Science, McGill Univ., 21111 Lakeshore, Ste-Anne-de-Bellevue, QC, Canada, H9X 3V9
^b Grain Research Laboratory, 1404-303 Main St., Winnipeg, MB, Canada R3C 3G8
^c Crop Development Centre, Univ. of Saskatchewan, Saskatoon, SK, Canada S7N 5A8
^d Agriculture and Agri-Food Canada, P.O. Box 39088, St. John's, NF, Canada A1E 5Y7
^e Agriculture and Agri-Food Canada, Brandon, MB, Canada R7A 5Y3
^f Dep. of Plant Sciences, Univ. of Saskatchewan, Saskatoon, SK, Canada S7N 5A8
^g Monsanto, 67 Scurfield Blvd., Winnipeg, MB, Canada R3Y 1G4
^h Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, Ottawa, ON, Canada K1A 0C6
ⁱ Dep. of Agricultural, Food and Nutritional Science, Univ. of Alberta, Edmonton, AB, Canada T6G 2P5
^j Dep. of Plant Agriculture, Univ. of Guelph, Guelph, ON Canada, N1G 2W1

mather{at}macdonald.mcgill.ca

Malt quality traits of barley (Hordeum vulgare L.) may be suitable candidates for marker-assisted selection, as their evaluation involves laborious and costly procedures. Four regions of the genome were previously reported to affect several grain and malt quality traits in the two-row barley cross `Harrington'/`TR306'. This study used an independent set of lines derived from the same cross to verify the existence of quantitative trait loci (QTL) in these regions. Molecular marker genotypes were used to select 47 lines from among 410 Harrington/TR306 lines that had not been used in the original mapping experiment. Four groups of lines were selected on the basis of their genotype at marker loci in two regions on chromosome 7 (5H) with QTL affecting kernel weight and plumpness, grain protein, extract ß-glucan content, the difference between fine-grind and coarse-grind extract, soluble protein, diastatic power, -amylase activity and fine-grind extract, and in regions on chromosomes 3 (3H) and 6 (6H) with QTL affecting extract ß-glucan content and fine-coarse difference. Grain and malt quality traits of these lines were determined from grain grown in five field environments in western Canada. The results confirmed the presence of QTL on chromosome 7 affecting all traits previously reported. Marker-based selection for two regions on chromosome 7 was effective in identifying phenotypically superior lines, and the magnitudes of the combined effects for these regions were close to the estimates calculated in the mapping experiment. The presence of QTL on chromosomes 3 and 6 could not be confirmed as categorically, but combined selection for extract ß-glucan content and fine-coarse difference at all four QTL regions was more effective than selection for only the two regions on chromosome 7.

Abbreviations: centimorgan, cM • QTL, quantitative trait locus or loci • DH, doubled haploid • RFLP, restriction fragment length polymorphism

This article has been cited by other articles:

				T. Ishii and K. Yonezawa Optimization of the Marker-Based Procedures for Pyramiding Genes from Multiple Donor Lines: II. Strategies for Selecting the Objective Homozygous Plant Crop Sci., September 1, 2007; 47(5): 1878 - 1886. [Abstract] [Full Text] [PDF]

				R. Mihaljevic, H. F. Utz, and A. E. Melchinger Congruency of Quantitative Trait Loci Detected for Agronomic Traits in Testcrosses of Five Populations of European Maize Crop Sci., January 1, 2004; 44(1): 114 - 124. [Abstract] [Full Text] [PDF]

				M. Ayoub, E. Armstrong, G. Bridger, M. G. Fortin, and D. E. Mather Marker-Based Selection in Barley for a QTL Region Affecting {alpha}-Amylase Activity of Malt Crop Sci., March 1, 2003; 43(2): 556 - 561. [Abstract] [Full Text] [PDF]