HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (26) Citing Articles via Google Scholar Google Scholar Articles by Zimnoch-Guzowska, E. Articles by Gebhardt, C. Search for Related Content PubMed Articles by Zimnoch-Guzowska, E. Articles by Gebhardt, C. Agricola Articles by Zimnoch-Guzowska, E. Articles by Gebhardt, C.

CELL BIOLOGY & MOLECULAR GENETICS

QTL Analysis of New Sources of Resistance to Erwinia carotovora ssp. atroseptica in Potato Done by AFLP, RFLP, and Resistance-Gene-Like Markers

^a Plant Breeding and Acclimatization Institute, Mlochów Research Center, 05-832 Rozalin, Poland
^b Max-Planck Institute for Breeding Research, Carl von Linne Weg 10, D-50829 Köln, Germany

gebhardt{at}mpiz-koeln.mpg.de

The two most important bacterial diseases of cultivated potato, blackleg of stems and tuber soft rot, are caused by Erwinia species. Genetic resistance currently present in cultivars is insufficient to protect the crop. New sources of polygenic resistance to Erwinia carotovora ssp. atroseptica (van Hall) Dye (Eca) have been selected in diploid hybrids originating from intercrossing Solanum tuberosum L with the wild species S. chacoense Bitter and S. yungasense Hawk. One F1 hybrid population derived from these materials was used to locate, on the molecular map of potato, quantitative trait loci (QTL) for resistance of tubers and leaves to Eca. A linkage map was constructed based on AFLP and RFLP markers including three resistance-gene-like (RGL) markers. Clustering of AFLP markers in putative centromeric regions was observed. QTL analysis revealed complex inheritance of resistance to Eca. Genetic factors affecting resistance to Eca were located on all 12 potato chromosomes. Putative QTL for tuber resistance were identified on 10 chromosomes. The QTL with the largest and most reproducible effect on tuber resistance mapped to chromosome I. Effects on leaf resistance were less reproducible than effects on tuber resistance. Putative QTL for leaf resistance were identified on 10 chromosomes. Inheritance of tuber and leaf resistance to Eca was largely independent. Several QTL for resistance to Eca were linked to RGL loci. Four of those QTL mapped to genomic segments that have been shown to contain factors for qualitative and quantitative resistance to different pathogens in potato, tomato (Lycopersicon esculentum Mill.), or tobacco (Nicotiana tabacum L.).

Abbreviations: AFLP, amplified fragment length polymorphism • cfu, colony forming units • GLM, general linear model • LG, linkage group • QTL, quantitative trait locus • RFLP, restriction fragment length polymorphism • RGL, resistance gene like

This article has been cited by other articles:

				L. Li, J. Strahwald, H.-R. Hofferbert, J. Lubeck, E. Tacke, H. Junghans, J. Wunder, and C. Gebhardt DNA Variation at the Invertase Locus invGE/GF Is Associated With Tuber Quality Traits in Populations of Potato Breeding Clones Genetics, June 1, 2005; 170(2): 813 - 821. [Abstract] [Full Text] [PDF]