HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (14) Citing Articles via Google Scholar Google Scholar Articles by Corrêa, R. X. Articles by de Barros, E.G. Search for Related Content PubMed Articles by Corrêa, R. X. Articles by de Barros, E.G. Agricola Articles by Corrêa, R. X. Articles by de Barros, E.G.

CELL BIOLOGY & MOLECULAR GENETICS

Sequence Characterized Amplified Regions Linked to Rust Resistance Genes in the Common Bean

Departamento de Biologia Geral/BIOAGRO, UFV, 36571-000, Viçosa, MG, Brazil

ebarros{at}mail.ufv.br

Uromyces appendiculatus (Pers.) Unger, the causative fungus of rust in common beans (Phaseolus vulgaris L.), consists of many pathotypes or pathogenic races. Cultivar Ouro Negro is resistant to most pathotypes detected in Brazil in the last few decades. We attempted to identify molecular markers linked to a rust resistance gene block present in Ouro Negro. DNA samples extracted from homozygous near isogenic BC₃F_2:3 lines derived from a cross between susceptible cultivar US Pinto 111 and Ouro Negro were grouped, following greenhouse inoculations, into two contrasting bulks, one containing only resistant and the other only susceptible plants. The bulks were amplified with 605 random primers and two of them amplified bands which were heteromorphic between the two bulks. These random amplified polymorphic DNA (RAPD) bands were transformed into sequence characterized amplified regions (SCARs) SCARBA08 and SCARF10. The rust resistance–susceptibility phenotypes and the molecular genotypes with the two SCAR markers of 303 F_2:3 families from US Pinto 111 x Ouro Negro were determined. SCARBA08 and SCARF10 were determined to be 4.3 ± 1.2 and 6.0 ± 1.3 centimorgans (cM) from the rust resistance locus, respectively. These markers are being used in combination with other markers for resistance to rust, anthracnose, and angular leaf spot previously identified in our laboratory to aid the indirect selection of desirable plants in segregating populations in which Ouro Negro has been used as donor parent.

Abbreviations: ASAP, allele-specific associated primers • bp, base pairs • cM, centimorgan • NIL, near isogenic line • RAPD, random amplified polymorphic DNA • SCAR, sequence characterized amplified region

This article has been cited by other articles:

				S. O. Park, J. R. Steadman, D. P. Coyne, and K. M. Crosby Development of a Coupling-Phase SCAR Marker Linked to the Ur-7 Rust Resistance Gene and Its Occurrence in Diverse Common Bean Lines Crop Sci., January 16, 2008; 48(1): 357 - 363. [Abstract] [Full Text] [PDF]

				S. O. Park, D. P. Coyne, J. R. Steadman, K. M. Crosby, and M. A. Brick RAPD and SCAR Markers Linked to the Ur-6 Andean Gene Controlling Specific Rust Resistance in Common Bean Crop Sci., September 1, 2004; 44(5): 1799 - 1807. [Abstract] [Full Text] [PDF]