Crop Science Journal of Natural Resources and Life Sciences Education
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Crop Science 40:797-803 (2000)
© 2000 Crop Science Society of America

CELL BIOLOGY & MOLECULAR GENETICS

Progeny Analysis of Glyphosate Selected Transgenic Soybeans Derived from Agrobacterium-Mediated Transformation

Thomas E. Clementea, Bradley J. LaValleeb, Arlene R. Howeb, Dannette Conner-Wardb, Renee J. Rozmanb, Priscilla E. Hunterb, Debra L. Broylesb, Daniel S. Kastenb and Maud A. Hincheeb

a Plant Transformation Core Research Facility, E324, The Beadle Center, Univ. of Nebraska, Lincoln, NE 68588-0665 USA
b Monsanto Company, 700 Chesterfieldvillage Parkway North, St. Louis, MO 63198 USA

tclement{at}unlnotes.unl.edu

Glyphosate selection has been implemented into an Agrobacterium-mediated transformation system for soybean [Glycine max (L.) Merr.]. Cotyledonary explants were excised from 5-d-old seedlings and inoculated with an Agrobacterium strain carrying the CP4 gene, which confers tolerance to the herbicide Roundup, and the ß-glucuronidase (GUS) gene. Explants were selected on sublethal levels of glyphosate during shoot initiation and elongation. Glyphosate tolerant shoots were identifiable after 8 to 12 wk of selection. Coexpression of GUS occurred in approximately 80% of the primary transformants (R0). The R0 plants were fully fertile, with no apparent phenotypic abnormalities. Progeny analysis from 81 independent transgenic soybean events derived from glyphosate selection demonstrated that the majority of the transformants transmitted the transgenes as a single functional locus.

Abbreviations: AS, acetosyringone • BAP, 6-benzylaminopurine • GA3, gibberellic acid • GUS, ß-glucuronidase • RT, Roundup tolerance




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