HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Hegstad, J.M. Articles by Nickell, C.D. Search for Related Content PubMed Articles by Hegstad, J.M. Articles by Nickell, C.D. Agricola Articles by Hegstad, J.M. Articles by Nickell, C.D.

CELL BIOLOGY & MOLECULAR GENETICS

Positioning the wp Flower Color Locus on the Soybean Genome Map

^a Univ. of Illinois at Urbana-Champaign, Dep. of Crop Sciences, 1102 S. Goodwin, Urbana, IL 61801 USA

cnickell{at}uiuc.edu

The wp allele generates a pink flowered phenotype in soybean [Glycine max (L.) Merr.]. Genotypes with W1_wp wp are associated with larger seed size and higher seed protein content. The influence of a single locus on the anthocyanin pathway and seed protein accumulation has not been documented in other species. It is of interest to clone wp by initially identifying linked molecular markers. The objective of this research was to use restriction fragment length polymorphism (RFLP) and simple sequence repeat (SSR) molecular markers to position the wp locus on the soybean public genetic map. Of the 74 markers tested, two RFLP and 14 SSR markers were identified as linked to wp. The two RFLP markers linked to wp in this study were previously reported to be associated with quantitative trait loci (QTL) controlling seed protein in a G. soja Siebold & Zucc. x G. max population. It is proposed that wp is located between RFLP locus K011 and SSR locus Satt600. The molecular markers linked to wp were located on linkage group D1b+W of the public soybean genetic map. The knowledge of linked molecular markers will assist in cloning wp, potentially leading to further characterization of how wp influences the anthocyanin pathway and seed protein accumulation.

Abbreviations: AFLP, amplified fragment length polymorphism • cM, centimorgan • PCR, polymerase chain reaction • QTL, quantitative trait loci • RAPD, random amplified polymorphic DNA • RFLP, restriction fragment length polymorphism • SSR, simple sequence repeat

This article has been cited by other articles:

				G. Zabala and L. O. Vodkin The wp Mutation of Glycine max Carries a Gene-Fragment-Rich Transposon of the CACTA Superfamily PLANT CELL, October 1, 2005; 17(10): 2619 - 2632. [Abstract] [Full Text] [PDF]

				K. D. Burnham, A. E. Dorrance, T. T. VanToai, and S. K. St. Martin Quantitative Trait Loci for Partial Resistance to Phytophthora sojae in Soybean Crop Sci., September 1, 2003; 43(5): 1610 - 1617. [Abstract] [Full Text] [PDF]

				H. C. Karakaya, Y. Tang, P. B. Cregan, and H. T. Knap Molecular mapping of the fasciation mutation in soybean, Glycine max (Leguminosae) Am. J. Botany, April 1, 2002; 89(4): 559 - 565. [Abstract] [Full Text] [PDF]

				A.M. Sebolt, R.C. Shoemaker, and B.W. Diers Analysis of a Quantitative Trait Locus Allele from Wild Soybean That Increases Seed Protein Concentration in Soybean Crop Sci., September 1, 2000; 40(5): 1438 - 1444. [Abstract] [Full Text]