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Published in Crop Sci 39:1518-1521 (1999)
© 1999 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Crop Science 39:1518-1521 (1999)
© 1999 Crop Science Society of America

NOTES

Nuclear dna content of thirteen turfgrass species by flow cytometry

K. Arumuganathan1,a, S.P. Tallury1,b, M.L. Fraserc, A.H. Bruneaub and R. Qub

a Center for Biotechnology, Univ. of Nebraska, Lincoln, NE 68588 USA
b Dep. of Crop Science, North Carolina State Univ., Raleigh, NC 27695 USA
c Pure Seed Testing, Inc., Rolesville, NC 27571 USA

rongda_qu{at}ncsu.edu

Knowledge of nuclear DNA content will facilitate future molecular studies of turfgrasses. The objective of this study was to determine genome size of 12 turfgrass species and an interspecific hybrid by means of flow cytometry and to compare genome size of warm and cool-season grasses. The seven species and an interspecific hybrid of warm-season turfgrasses had genome sizes ranging from 0.86 to 1.95 pg/2C, while the genome sizes of five cool-season grasses ranged from 5.65 to 15.59 pg/2C. Ploidy levels of the samples were also determined. The observation of the distinct genome sizes of the warm and cool-season turfgrasses agrees with previous reports regarding genome sizes of tropical and temperate species in certain angiosperm families including Gramineae.

Abbreviations: C, DNA content of the non-replicated haploid chromosome complement




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