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CELL BIOLOGY & MOLECULAR GENETICS

Confirmation of QTL Associated with Common Bacterial Blight Resistance in Four Different Genetic Backgrounds in Common Bean

^a Dep. of Horticulture, Univ. of Wisconsin, Madison, WI 53706 USA
^b Washington Univ., School of Medicine, Dep. of Biochemistry and Molecular Biophysics, St. Louis, MO 63110 USA
^c Dep. of Horticulture, Univ. of Nebraska, Lincoln, NE 68583 USA
^d Centro de Investigaciones Agricola del Surocate (CIAS), San Juan de la Maguana, Dominican Republic

gjung{at}facstaff.wisc.edu

Common bacterial blight (CBB), incited by the bacterial pathogen Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp) is a major problem in bean (Phaseolus vulgaris L.) producing areas worldwide. Using 128 recombinant inbred (RI) lines derived from the common bean cross BAC 6 x HT 7719, RAPD marker locus-QTL associations were previously described for resistance to two Xcp strains, EK-11 and Epif-IV. The objective of this research was to test these candidate marker locus-QTL associations in three previously untested genetic populations. In addition, RAPD marker locus-QTL associations were also investigated for resistance to a third Xcp strain, DR-7, in the first trifoliolate leaves in the original BAC 6 x HT 7719 population. The three genomic regions most significantly associated with CBB resistance in the original BAC 6 x HT 7719 population were significantly associated with CBB resistance in at least two of the three additional populations. The unmapped marker, BC409.1250, was significantly associated with CBB resistance in all four populations and all three Xcp strains, suggesting that this marker might be tightly linked to genes for CBB resistance. The RAPD marker BC409.1250 was converted into a marker that is a robust and reliable PCR-based marker. Since similar genomic regions were found for resistance to three different Xcp strains, these QTL may be useful for breeding cultivars with a broad range of resistance.

Abbreviations: CBB, common bacterial blight • cM, centimorgan • FL, first trifoliolate leaves • GLS, gray leaf spot • GN, great northern • LDL, later developed trifoliolate leaves • LOD, log odds ratio • PCR, polymerase chain reaction • QTL, quantitative trait locus (loci) • RAPD, random amplified polymorphic DNA • RIL, recombinant inbred lines • SCAR, sequence characterized amplified regions • SSD, single seed descent • Xcp, Xanthomonas campestris pv. phaseoli

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