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Published in Crop Sci 39:248-252 (1999)
© 1999 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Verifications of a Quantitative Trait Locus Affecting Agronomic Traits in Two-Row Barley

D. Spaner, B. G. Rossnagel and W. G. Legge

Agriculture and Agri-Food Canada, St. John's, NF A1E 5Y7, Canada
Crop Development Centre, Univ. of Saskatchewan, Saskatoon, SK S7N 5A8, Canada
Agriculture and Agri-Food Canada, Brandon MB R7A 5Y3, Canada

G. J. Scoles and P. E. Eckstein

Dep. of Plant Sciences, Univ. of Saskatchewan, Saskatoon, Sk S7N 5A8, Canada

G. A. Penner, N. A. Tinker and K. G. Briggs

Agriculture and Agri-Food Canada, Winnipeg, MB R3T 2M9, Canada
Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada
Dep. of Agriculture, Food and Nutritional Science, Univ. of Alberta, Edmonton AB T6G 2P5, Canada

D. E. Falk and J. C. Afele

Dep. of Plant Agriculture, Univ. of Guelph, Guelph ON N1G 2W1, Canada

P. M. Hayes and D. E. Mather*

Dep. of Crop and Soil Science, Oregon State Univ., Corvallis OR 97331
Dep. of Plant Science, McGill Univ., 21111 Lakeshore, Ste-Anne-Bellevue, QC H9X 3V9, Canada

* Corresponding author (mather{at}agradm.lan.mcgill.ca).

Quantitative trait loci (QTL) affecting agronomic traits have been mapped in barley (Hordeum vulgare L.), but there have been few experimental verifications of the presence and effect of individual QTL. Here, we report on verification of a QTL on the "plus" arm of chromosome 7(5H) of barley. This QTL, affecting grain yield, plant height, maturity, and lodging severity, had been mapped in the two row barley cross ‘Harrington’/‘TR306’, on the basis of evaluation of 145 doubled haploid lines in 30 field experiments. We used molecular marker genotypes to select 47 lines from among 410 Harrington/TR306 lines that had not been used in the original mapping experiment. Comparisons among groups of lines with contrasting marker genotypes on chromosome 7 (5H) confirmed that a QTL on the "plus" arm of that chromosome affects grain yield, plant height, and lodging severity. The presence of TR306 alleles at this QTL contributed to small increases in grain yield and plant height, and reduced lodging severity. The results of this study confirmed the presence of a QTL affecting several agronomic traits, but also demonstrated that the effect of individual QTL may be small and may vary among environments.

Received for publication February 27, 1998.


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