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DNA-Based Markers for a Recessive Gene Conferring Anthracnose Resistance in Sorghum

Dep. of Biotechnology and Molecular Biology, Haryana Agric. Univ., Hisar 125004, India

Richard Frederiksen and Clint Magill^*

Dep. of Plant Pathology and Microbiology, Texas A&M Univ., College Station, TX 77843

^* Corresponding author (c-magill{at}tamu.edu).

Resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench] accession SC326-6 was found to segregate as a simple recessive trait in a cross to the susceptible cultivar BTx623.F3 progeny tests following self pollination of 115 F₂ individuals identified homozygous resistant and susceptible F₂ plants for use in bulked-segregant analysis. DNA from the parental cultivars and the bulks was screened by PCR amplification with 300 RAPD (random amplified polymorphic DNA) primers. Two RAPD primers amplified a sequence that co-segregated with the recessive resistance allele, while another amplified a band linked to the susceptible allele.

Received for publication November 23, 1997.