HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gourmet, C. Articles by Rayburn, A. L. Search for Related Content PubMed Articles by Gourmet, C. Articles by Rayburn, A. L. Agricola Articles by Gourmet, C. Articles by Rayburn, A. L.

The Nonselective Nature of a Modified Single-Seed Descent Breeding Procedure

Dep. of Agronomy, Univ. of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706

F. L. Kolb, C. M. Brown and C. A. Smyth

1102 S. Goodwin Ave., Dep. of Crop Sciences, Univ. of Illinois, Urbana, IL 61801

D. P. Biradar and A. L. Rayburn^*

Dep. of Agric. Sciences, Krishinagar, Dharwar 580 005, Karnataka, India
1201 West Gregory Drive

^* Corresponding author (arayburn{at}uiuc.edu).

The objectives of this study were to determine (i) if selection for barley yellow dwarf virus (BYDV) tolerance occurred under a modified single-seed descent (MSSD) procedure when used in conjunction with BYDV inoculation, and (ii) if changes in nuclear DNA content occurred during MSSD. Plants from four intraspecific spring oat (Avena sativa L.) crosses were grown in the greenhouse under a MSSD procedure and were infected with BYDV by inoculation with viruliferous aphids. These populations were compared with populations grown without exposure to BYDV. BYDV tolerance was evaluated in the field in the F_4:5 and F_4:6 generations. Inoculation with BYDV under the MSSD method did not selectively eliminate BYDV susceptible genotypes. To determine if the MSSD procedure induced selection for a particular nucleotype, seeds harvested from the parents (grown under the MSSD procedure) were tested for nuclear DNA content. No difference in DNA content was observed among progeny grown from seeds of stressed plants compared with progeny grown from seeds of nonstressed plants. These results suggest that even the most BYDV susceptible plants produced seeds during MSSD, and the MSSD method does not seem to induce directed changes in nuclear DNA content.

Received for publication May 6, 1996.