Crop Science Grow Your Career with CSSA
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Published in Crop Sci 37:595-598 (1997)
© 1997 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hultquist, S. J.
Right arrow Articles by Kaeppler, S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hultquist, S. J.
Right arrow Articles by Kaeppler, S.
Agricola
Right arrow Articles by Hultquist, S. J.
Right arrow Articles by Kaeppler, S.

DNA Content and Chloroplast DNA Polymorphisms among Switchgrasses from Remnant Midwestern Prairies

Sherry J. Hultquist, K. P. Vogel*, D. J. Lee, K. Arumuganathan and S. Kaeppler

Agronomy Dep., Univ. of Nebraska, Lincoln NE
USDA-ARS, 344 Keim Hall, Univ. of Nebraska, P.O. Box 830937, Lincoln, NE 68583-0937and Center for Grassland Studies, Univ. of Nebraska, Lincoln, NE
Center for Biotechnology, Univ. of Nebraska, Lincoln NE
Dep. of Agronomy, Univ. of Wisconsin, Madison, WI 53706

* Corresponding author (E-mail: AGROO12{at}UNLVM.UNL.EDU).

Panicum virgatum L. (switchgrass) is perennial grass that is native to most of the USA. The principal use of switchgrass has been as a pasture and range grass for forage production during the warmer summer months. The objective of this research was to determine if DNA content and chloroplast DNA (cpDNA) polymorphisms existed among and within switchgrass accessions from Midwestern prairie sites. Twenty-eight switchgrass accessions from remnant prairie sites were surveyed in 1994 for the upland cytotype (U) or lowland (L) cytotype in the cpDNA by means of restriction endonuclease BamHI and sorghum cpDNA probe pLD 5. These accessions were also surveyed in 1994 for DNA content differences ltow cytometry. One accession, IA 31, contained a mixture of cytotypes indicating that both cytotypes can occur at a single remnant prairie site. The other Midwestern remnant prairies that were represented in this study contained only the U cytotype. The flow cytometry results indicated that switchgrass populations found within the Midwestern sites can be a mixture of ploidy levels with either 3 (tetraploid) or 6 (octaploid) pg DNA per cell. These results indicated that germplasm from Midwestern prairies should be identified according to DNA content and cytotype before it is utilized in developmental programs by plant breeders.


The reported research is from a dissertation submitted by the senior author (Sherry Jean Hultquist Elmore) in partial fulfillment of the requirements for a Ph.D. degree at the University of Nebraska. The research was funded in part by the U.S. Dep. of Energy's Biomass Fuels program via Oak Ridge National Laboratory, USDA-ARS, and the University of Nebraska. Contract no. DE-A105-900R21954. Journal series no. 11277. Nebraska Agricultural Experiment Station.




This article has been cited by other articles:


Home page
Crop Sci.Home page
J. M. Martinez-Reyna and K. P. Vogel
Heterosis in Switchgrass: Spaced Plants
Crop Sci., July 1, 2008; 48(4): 1312 - 1320.
[Abstract] [Full Text] [PDF]


Home page
Crop Sci.Home page
O. Gulsen, R. C. Shearman, T. M. Heng-Moss, N. Mutlu, D. J. Lee, and G. Sarath
Peroxidase Gene Polymorphism in Buffalograss and Other Grasses
Crop Sci., March 1, 2007; 47(2): 767 - 772.
[Abstract] [Full Text] [PDF]


Home page
Crop Sci.Home page
O. Gulsen, R. C. Shearman, K. P. Vogel, D. J. Lee, and T. Heng-Moss
Organelle DNA Diversity among Buffalograsses from the Great Plains of North America Determined by cpDNA and mtDNA RFLPs
Crop Sci., January 1, 2005; 45(1): 186 - 192.
[Abstract] [Full Text] [PDF]


Home page
Crop Sci.Home page
M. D. Casler
Ecotypic Variation among Switchgrass Populations from the Northern USA
Crop Sci., January 1, 2005; 45(1): 388 - 398.
[Abstract] [Full Text] [PDF]


Home page
Crop Sci.Home page
M. D. Casler, K. P. Vogel, C. M. Taliaferro, and R. L. Wynia
Latitudinal Adaptation of Switchgrass Populations
Crop Sci., January 1, 2004; 44(1): 293 - 303.
[Abstract] [Full Text] [PDF]


Home page
Crop Sci.Home page
A. Boe
Genetic and Environmental Effects on Seed Weight and Seed Yield in Switchgrass
Crop Sci., January 1, 2003; 43(1): 63 - 67.
[Abstract] [Full Text] [PDF]


Home page
Crop Sci.Home page
J. M. Martinez-Reyna and K. P. Vogel
Incompatibility Systems in Switchgrass
Crop Sci., November 1, 2002; 42(6): 1800 - 1805.
[Abstract] [Full Text] [PDF]


Home page
Crop Sci.Home page
M. Tuna, K. P. Vogel, K. Arumuganathan, and K. S. Gill
DNA Content and Ploidy Determination of Bromegrass Germplasm Accessions by Flow Cytometry
Crop Sci., September 1, 2001; 41(5): 1629 - 1634.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Bot.Home page
K. H. Keeler and G. A. Davis
Comparison of common cytotypesof Andropogon gerardii(Andropogoneae, Poaceae)
Am. J. Botany, July 1, 1999; 86(7): 974 - 979.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
The SCI Journals Agronomy Journal Vadose Zone Journal
Journal of Natural Resources
and Life Sciences Education
Soil Science Society of America Journal
Journal of Plant Registrations Journal of
Environmental Quality
The Plant Genome
Copyright © 1997 by the Crop Science Society of America.