HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Adcock, R. A. Articles by Ware, G. O. Search for Related Content PubMed Articles by Adcock, R. A. Articles by Ware, G. O. Agricola Articles by Adcock, R. A. Articles by Ware, G. O.

Sample Variation and Resource Allocation for Ergot Alkaloid Characterization in Endophyte-Infected Tall Fescue

4705 Mountain Crest Dr., Guntersville, AL 35976

N. S. Hill^* and H. R. Boerma

Dep. of Crop and Soil Science, Univ. of Georgia, Athens, GA 30602

G. O. Ware

College of Agriculture and Environmental Sciences, Univ. of Georgia, Athens, GA 30602

^* Corresponding author (nhill{at}uga.cc.uga.edu).

Ergot alkaloid concentrations vary among genotypes of endophyteinfected (E + ) tall fescue (Festuca arundinacea Schreb.). Because the ergot alkaloids are responsible for detrimental health disorders in grazing livestock, but the endophyte Neotyphodium coenophialum (Morgan-Jones and Gams) Glenn, Bacon and Hanlin is mutualistic, development of E + tall fescue populations low in ergot alkaloid concentration may be a sustainable non-toxic solution for livestock producers. Current techniques for screening ergot alkaloids in tall rescue are labor and equipment intensive and time consuming. The objective of this research was to determine the number of replications and locations needed to detect specified differences among treatments varying in alkaloid concentration. Six E + tall fescue genotypes were harvested twice at two field locations and one greenhouse location in 1 yr, and the number of replications and environments required to show specific treatment differences for alkaloid concentration calculated within harvest dates. Ergot alkaloid concentrations for the genotypes ranged from 488 mg kg^–1 to 2504 mg kg^–1 in the spring, and 219 mg kg^–1 to 986 mg kg^–1 in the summer. Genotypic variances were proportional to the mean; hence, data were subjected to a log₁₀ transformation. Treatments harvested twice with differences of 50% of the experimental mean were detectable (P = 0.05) in experiments containing two replications grown at four environments, or two replications grown at three environments and harvested once when nontransformed or transformed data were used, respectively. Use of transformed data will be necessary to keep the number of observations per experimental unit manageable.

Received for publication October 11, 1995.

This article has been cited by other articles:

				N. S. Hill, S. M. Neate, B. Cooper, R. Horsley, P. Schwarz, L. S. Dahleen, K. P. Smith, K. O'Donnell, and J. Reeves Comparison of ELISA for Fusarium, Visual Screening, and Deoxynivalenol Analysis of Fusarium Head Blight for Barley Field Nurseries Crop Sci., July 1, 2008; 48(4): 1389 - 1398. [Abstract] [Full Text] [PDF]