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Micropropagation of Switchgrass by Node Culture

The University of Tennessee, Dep. of Plant and Soil Science, Knoxville, TN 37901-1071

^* Corresponding author (congerbv{at}utk.edu).

Switchgrass (Panicum virgatum L.) is naturally outcrossing; therefore, maintenance of genotype is difficult through sexual propagation. The objective of this study was to develop a micropropagation procedure for the multiplication of desired or selected genotypes. Nodal segments were surface sterilized, split longitudinally, and placed with the cut surface on solid MS medium containing 30 g L^–1 maltose and different concentrations of 6-benzylaminopurine (BAP) as the only growth regulator. Best shoot proliferation was obtained with 12.5 µM BAP and culture at 29°C. Shoots were easily rooted on MS medium without BAP. Under optimum conditions, it is possible to produce approximately 500 plantlets from one parent plant in 12 wk.

Received for publication January 23, 1996.

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