Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 36:1049-1052 (1996)
© 1996 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, Panicum virgatum L.

Sherry J. Hultquist, K. P. Vogel*, D. J. Lee, K. Arumuganathan and S. Kaeppler

Dep. of Agronomy, Univ. of Nebraska, Lincoln, NE
USDA-ARS, 344 Keim Hall, Univ. of Nebraska, P.O. Box 830937, Lincoln, NE 68583-0937and Center for Grassland Studies, Univ. of Nebraska, Lincoln, NE
Center for Biotechnology, Univ. of Nebraska, Lincoln, NE 68583-0665

* Corresponding author (agro012{at}unlvm.unl.edu).

Switchgrass, Panicum virgatum L., is a native, cross-pollinated, morphologically diverse species with an array of ploidy levels and ecotypes. Switchgrass is found throughout most of the USA and Canada, primarily east of the Rocky Mountains and south of Hudson Bay. The objective of this research was to determine if chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) occur among switchgrass cultivars and experimental strains that differ in ploidy level or ecotype classification. Eighteen switchgrass cultivars or experimental strains representative of reported ecotypes, ploidy levels, and the geographical range of switchgrass were surveyed for cpDNA polymorphisms by means of four restriction endonucleases and 20 sorghum cpDNA probes. One polymorphism was detected which was associated with the lowland-upland ecotype classification. The lowland cultivars contained a restriction site change that was not present in the upland cultivars. The two cytotypes discovered have been designated as the U (upland) or L (lowland) cytotype. The lowland cultivars had 3 pg DNA/nuclei as measured by flow cytometry while the upland types had either 3 or 6 pg DNA/nuclei. There were no cpDNA polymorphisms among the upland cultivars regardless of ploidy level as measured by DNA content. These results demonstrate that cpDNA differs among switchgrasses and that this variation is associated with ecotype variation but not with nuclear DNA content.

The reported research is from a dissertation submitted by the senior author (Sherry Jean Hulquist Elmore) in partial fulfillment of the requirements for a Ph.D. degree at the University of Nebraska. The research was funded in part by the U.S. Dept. of Energy's Biomass Fuels program via Oak Ridge National Laboratory, USDA-ARS, and the University of Nebraska. Contract no. DE-A105-900R21954. Journal series no. 11278. Nebraska Agricultural Experiment Station.




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