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Published in Crop Sci 35:1400-1405 (1995)
© 1995 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Evaluating Wheat Nitrogen Status with Canopy Reflectance Indices and Discriminant Analysis

I. Filella*, L. Serrano, J. Serra and J. Peñuelas

CREAF i Centre de Referència d'Estudis Ambientals, Facultat de Ciències, Universitat Autònoma, 08193 Bellaterra, Barcelona, Spain
Fundació Mas Badia, E-17134 La Tallada (Girona), Spain

* Corresponding author (penuelas{at}cc.uab.es).

Nitrogen deficiencies can seriously reduce yield and economic returns for farmers. Remote sensing could provide inexpensive, largearea estimates of N status and be used to monitor N status since leaf chlorophyll (Chi) A content is mainly determined by N availability. The objective was to determine if remote sensing of wheat (Triticum aestivum L.) Chi A content would provide a rapid estimation of wheat N status. We measured the reflectance of a wheat crop submitted to five different fertilization treatments throughout the growth cycle. We tested several empirical reflectance indices of pigment content: reflectance at 550 nm (R550), reflectance at 680 nm (R680), three parameters of the red edge [wavelength ({lambda}re), amplitude in the first derivative of the reflectance spectra (dRre), and sum of amplitudes between 680 and 780 nm in the first derivative of the reflectance spectra ({Sigma}dR680–780nm)], and pigment simple ratio (PSR) and normalized pigment chlorophyll index (NPCI) (indices of carotenoid/Chl ratio). We also measured leaf Chi A and N content, and leaf area index. There were significant correlations between canopy Chi A content and R550, R680, and all the red edge parameters. The NPCI and PSR followed phenological evolution of the carotenoids/Chl A ratio and separated the different treatments. By discriminant analysis based on the pigment indices reflectance at 430 nm (R430), R550, R680, {lambda}re, dRre, and NPCI, each reflectance spectrum can be assigned to a different N status class. Thus, the use of these optical techniques offers a potential for assessing N status of wheat.

Received for publication September 13, 1994.


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