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Production and Identification of Chromosome Specific RAPD Markers for Langdon Durum Wheat Disomic Substitution Lines

USDA-ARS-FRRL, Utah State Univ., Logan, UT 84322-6300
Shaanxi Academy of Agric. Sci., Yangling, Shaanxi P.R., China
USDA-ARS-CCR, Fargo, ND 58105-5677

^* Corresponding author (rrcwang{at}cc.usu.edu).

Random amplified polymorphic DNA (RAPD) analyses were performed with DNA extracted from a complete set of Langdon durum wheat (Triticum turgidum L. var. durum) disomic substitution lines and their parents (Langdon and ‘Chinese Spring’) with the objective of assigning chromosomal locations for RAPD markers. Seven out of 12 arbitrary decamer primers yielded 11 products that could be assigned to a specific chromosome of the A- or B-genome from Langdon durum or to a D-genome chromosome of Chinese Spring. The 11 RAPD markers were assigned to three A-genome, five B-genome, and one D-genome chromosome. Chinese Spring ditelosomic lines were used to assign four RAPD markets to specific chromosome arms. Most of the markers were present in both Chinese Spring and Langdon, but one was genotype specific (e.g., J11c was present on Langdon chromosome 1B only). These reproducible RAPD markers of specific wheat chromosomes are additional tools for genome mapping.

Received for publication August 10, 1994.