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Quantifying Acquired Thermal Tolerance in Winter Wheat

USDA-ARS, Plant Science and Water Conservation Lab., 1301 N. Western St., Stillwater, OK 74075
Dep. of Agronomy, Horticulture, and Entomology, Texas Tech Univ., Lubbock, TX 79401
USDA-ARS, Cropping Systems Research Lab., Lubbock, TX 79401

^* Corresponding author.

High temperature stress limits productivity of winter wheat (Triticum aestivum L.) in many environments. Identification of thermal tolerant germplasm is needed to improve plant productivity under heat stress. In this study we used 2,3,5-triphenyltetrazolium chloride OWC) reduction, an indicator of mitochondrial activity, to characterize cultivar differences for acquired thermal tolerance in winter wheat. Seven-day-old seedlings of six hard red winter wheat cultivars grown at 25°C were aeelimated at 37°C for 24 h followed by a 50°C incubation for 2 h. Leaf sections sampled after each temperature treatment were infiltrated with TTC and the resultant formazan was ethanol-extracted for optical density measuremenatt 530 nm. Under these test conditions, the TTC assay detected differential tolerance (expressed as percent cell viability of the 50°C treated tissue compared to the 37°C treated tissue) which ranged from a low of 6% for ‘Siouxland’, to a high of 72% for ‘TAM 108’. The results of this study show that TTC reduction can be used to measure differences in acquired thermal tolerance in winter wheat.

Received for publication December 6, 1993.

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				A. M. H. Ibrahim and J. S. Quick Heritability of Heat Tolerance in Winter and Spring Wheat Crop Sci., September 1, 2001; 41(5): 1401 - 1405. [Abstract] [Full Text] [PDF]