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Immunological Identification of Seed Lipoxygenase Genotypes in Soybean

Dep. of Plant Science, Univ. of Adelaide, Waite Campus, Glen Osmond, Australia

W. E. Nyquist and J. B. Santini

Dep.of Agronomy, Purdue Univ., W. Lafayette, IN 47907-1150

Peter Bretting and N. C. Nielsen^*

USDA-ARS, NCRPIS, Dep. of Agronomy, Iowa State Univ., Ames, IA 50011
USDA-ARS, Dep. of Agronomy, Purdue Univ., West Lafayette, IN 47907-1150

^* Corresponding author (nnielsen{at}dept.agry.purdue.edu).

Elimination of lipoxygenases from soybean reduces the undesirable beany off-flavor associated with soy products. Breeding programs, as well as quality control procedures, that utilize lipoxygenase null-alleles to improve soybean flavor are dependent upon an accurate and efficient means to identify those lines that lack these enzymes. The use of an enzyme-linked immunosorbent assay is one approach to this problem. Monocional antibodies specific to each of the three predominant soybean [Glycine max. (L.) Merr] seed lipoxygenases are described. The antibodies can be used to quantitate the amount of each isozyme in seed extracts. The antibodies are useful for the identification of specific lipoxygenase genotypes in progeny from crosses among lines bearing lipoxygenase null-alleles. Statistical methodology is described that permits a determination of the percentage of contamination of L2L3-1ess seeds by normal seeds that contain lipoxygenases in seed lots derived from commercial operations. Statistical approaches to sampling such as this may have wider application to quality assurance in other identity preserved crops.

Received for publication January 18, 1993.