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Crop and Soil Science Dep., Univ. of Georgia, Athens, GA 30602
* Corresponding author.
Previously, a monoclonal antibody was developed to the lysergic ring common to ergoline alkaloids (EA). The objectives were to develop an immunoassay for EA in tall fescue by determining (i) time requirement; (ii) tissue mass, and (iii) optimum volume of extraction solution necessary for detection protocols with repeatable results; and (iv) the acceptability of oven- versus freeze-dried samples. A competitive enzyme-linked immunosorbent assay (ELISA) was conducted on plant extracts (0-548 µg kg–1 ergovaline). Regressions of ELISA and high performance liquid chromatography (HPLC) data gave good lits (R2 
0.87, MSE 
0.03). Using 0.10 g (±0.01 g) of oven-dried tissue, and extraction for 15 min in a 1:40 dilution, we analyze 200 samples daily.
Received for publication May 24, 1993.
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